[ PROPERTIES ]

Source: Prokaryotic expression.

Host: E. coli

Residues: Ser108~Pro343

Tags: Two N-terminal Tags, His-tag and GST-tag

Tissue Specificity: Brain.

Subcellular Location: Secreted, extracellular space, extracellular matrix.

Purity: >98%

Traits: Freeze-dried powder

Buffer formulation: PBS, pH7.4, containing 1mM DTT, 5% trehalose, 0.01% sarcosyl and Proclin300.

Original Concentration: 200ug/mL

Applications: SDS-PAGE; WB; ELISA; IP; CoIP; Purification; Amine Reactive Labeling.

(May be suitable for use in other assays to be determined by the end user.)

Predicted isoelectric point: 9.1

Predicted Molecular Mass: 56.0kDa

Accurate Molecular Mass: 56kDa as determined by SDS-PAGE reducing conditions.

[ USAGE ]

Reconstitute in PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

[ STORAGE AND STABILITY ]

Storage: Avoid repeated freeze/thaw cycles.

Store at 2-8oC for one month.

Aliquot and store at -80oC for 12 months.

Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

[ SEQUENCE ]

[ IDENTIFICATION ]

Figure 1. SDS-PAGE