Recombinant Dihydroorotate Dehydrogenase (DHODH)

Instruction Manual!

Product name:	Recombinant Dihydroorotate Dehydrogenase (DHODH)
Source:	E. coli
Purity:	> 90%
Buffer Formulation:	20mM Tris, 150mM NaCl, pH8.0, containing 0.01% SKL, 5% Trehalose
Applications:	Positive Control; Immunogen; SDS-PAGE; WB.
Storage:	Avoid repeated freeze/thaw cycles.Store at 2-8oC for one month.Aliquot and store at -80oC for 12 months.
UOM:	50µg/100µg/20µg/1mg/5mg

[ PROPERTIES ]

Source: Prokaryotic expression

Host: E.coli

Residues: Arg35~Asp392

Tags: N-terminal His Tag

Subcellular Location: Membrane

Purity: > 90%

Traits: Freeze-dried powder

Buffer formulation: 20mM Tris, 150mM NaCl, pH8.0, containing 0.01% SKL, 5% Trehalose.

Original Concentration: 200µg/mL

Applications: Positive Control; Immunogen; SDS-PAGE; WB.

(May be suitable for use in other assays to be determined by the end user.)

Predicted isoelectric point: 9.5

Predicted Molecular Mass: 42.4kDa

Accurate Molecular Mass: 42kDa as determined by SDS-PAGE reducing conditions.

[ USAGE ]

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

[ STORAGE AND STABILITY ]

Storage: Avoid repeated freeze/thaw cycles.

Store at 2-8ºC for one month.

Aliquot and store at -80ºC for 12 months.

Stability Test: The thermal stability is described by the loss rate. The loss rate was determinedby accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and noobvious degradation and precipitation were observed. The loss rate is less than 5% within theexpiration date under appropriate storage condition.

[ SEQUENCE ]

[ IDENTIFICATION ]

[ IMPORTANT NOTE ]

The kit is designed for research use only, we will not be responsible for any issue if the kit wasused in clinical diagnostic or any other procedures.