Recombinant Fibroblast Activation Protein Alpha (FAPa)

Instruction Manual!

Product name:	Recombinant Fibroblast Activation Protein Alpha (FAPa)
Source:	E. coli
Purity:	>95%
Buffer Formulation:	20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5%Trehalose
Applications:	Positive Control; Immunogen; SDS-PAGE; WB
Storage:	Avoid repeated freeze/thaw cycles.Store at 2-8oC for one month.Aliquot and store at -80oC for 12 months.
UOM:	50µg/100µg/200µg/1mg/5mg

[ PROPERTIES ]

Source: Prokaryotic expression.

Host: E. coli

Residues: Ile523~Asp760

Tags: N-terminal His-Tag

Tissue Specificity: Adipose, Fetal skin, Glioma, HSC.

Subcellular Location:Cell surface. Cell membrane; Single-pass type II membrane protein. Membrane, Single-pass type II membrane protein .

Purity: >95%

Traits: Freeze-dried powder

Buffer formulation: 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5%Trehalose.

Original Concentration: 200µg/mL

Applications: Positive Control; Immunogen; SDS-PAGE; WB.

(May be suitable for use in other assays to be determined by the end user.)

Predicted isoelectric point: 6.7

Predicted Molecular Mass: 30.5kDa

Accurate Molecular Mass: 26&40kDa as determined by SDS-PAGE reducing conditions.

Phenomenon explanation:

The possible reasons that the actual band size differs from the predicted are as follows:

Splice variants: Alternative splicing may create different sized proteins from the same gene.
Relative charge: The composition of amino acids may affects the charge of the protein.
Post-translational modification: Phosphorylation, glycosylation, methylation etc.
Post-translation cleavage: Many proteins are synthesized as pro-proteins, and then cleaved to give the active form.
Polymerization of the target protein: Dimerization, multimerization etc.

[ USAGE ]

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

[ STORAGE AND STABILITY ]

Storage: Avoid repeated freeze/thaw cycles.

Store at 2-8oC for one month.

Aliquot and store at -80oC for 12 months.

Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

[ SEQUENCE ]

[ IDENTIFICATION ]

Figure 1. Gene Sequencing (Extract)

Figure 2. SDS-PAGE