Recombinant Histone Deacetylase 6 (HDAC6)

Instruction Manual!

Product name:	Recombinant Histone Deacetylase 6 (HDAC6)
Source:	E. coli
Purity:	>95%
Buffer Formulation:	Supplied as lyophilized form in PBS, pH7.4, containing 5% sucrose, 0.01% sarcosyl.
Applications:	SDS-PAGE; WB; ELISA; IP
Storage:	Avoid repeated freeze/thaw cycles.Store at 2-8oC for one month.Aliquot and store at -80oC for 12 months.
UOM:	50µg/100µg/200µg/1mg/5mg

[ PROPERTIES ]

Figure 2. SDS-PAGE

Residues: Arg560~Pro811 (Accession # Q9Z2V5), with two N-terminal Tags, His-tag and T7-tag.

Host: E. coli

Subcellular Location: Nucleus. Cytoplasm.

Purity: >95%

Endotoxin Level: <1.0EU per 1μg (determined by the LAL method).

Formulation: Supplied as lyophilized form in PBS, pH7.4, containing 5% sucrose, 0.01% sarcosyl.

Predicted isoelectric point: 6.0 Predicted Molecular Mass: 30.6kDa Applications: SDS-PAGE; WB; ELISA; IP.

(May be suitable for use in other assays to be determined by the end user.)

[ FORMULATION ]

Reconstitute in sterile PBS, pH7.2-pH7.4

[ STORAGE AND STABILITY ]

Storage: Avoid repeated freeze/thaw cycles.

Store at 2-8oC for one month.

Aliquot and store at -80oC for 12 months.

Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

[ SEQUENCE ]

The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.

MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF-R EGANFDSIYI CPSTFACAKL ATGAACRLVE AVLSGEVLNG IAVVRPPGHH AEPNAACGFC FFNSVAVAAR HAQIIAGRAL RILIVDWDVH HGNGTQHIFE DDPSVLYVSL HRYDRGTFFP MGDEGASSQV GRDAGIGFTV NVPWNGPRMG DADYLAAWHR LVLPIAYEFN PELVLISAGF DAAQGDPLGG CQVTPEGYAH LTHLLMGLAG GRIILILEGG YNLASISESM AACTHSLLGD PPPQLTLLRP P