Recombinant Fibrillarin (FBL)

Instruction Manual!

Product name:	Recombinant Fibrillarin (FBL)
Source:	E.coli
Purity:	> 95%
Buffer Formulation:	20mM Tris, 150mM NaCl, pH8.0, containing 1mM
Applications:	SDS-PAGE; WB
Storage:	Avoid repeated freeze/thaw cycles.Store at 2-8oC for one month.Aliquot and store at -80oC for 12 months.
UOM:	50µg/100µg/20µg/1mg/5mg

[ PROPERTIES ]

Source: Prokaryotic expression

Organism Species: Homo sapiens (Human)

Host: E.coli

Residues: Gly37~Ala275

Tags: N-terminal His Tag Subcellular Location: Nucleus Purity: > 95%Traits: Freeze-dried powder

Buffer formulation: 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.

Original Concentration: 200µg/mL

Applications: Positive Control; Immunogen; SDS-PAGE; WB. (May be suitable for use in other assays to be determined by the end user.)

Predicted isoelectric point: 10.0

Predicted Molecular Mass: 28.7kDa

Accurate Molecular Mass: 29kDa as determined by SDS-PAGE reducing conditions.

[ USAGE ]

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0

mg/mL. Do not vortex.

[ STORAGE AND STABILITY ]

Storage: Avoid repeated freeze/thaw cycles.

Store at 2-8ºC for one month.

Aliquot and store at -80ºC for 12 months.

Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

[ SEQUENCE ]

[ IDENTIFICATION ]