[ PROPERTIES ]

Source: Natural Extract

Organism Species: Homo sapiens (Human)

Host: Human (Plasma)

Tissue Specificity: Plasma, Liver.

Subcellular Location: Secreted.

Purity: >92% as determined by SDS-PAGE.

Purification Methods: Salt co-precipitation and ionic-Exchange chromatography.

Traits: Freeze-dried powder

Buffer Formulation: PBS, pH7.4, containing 1mM DTT, 5% Trehalose and Proclin300.

Original Concentration: 200ug/mL

Applications: Positive Control; Immunogen; SDS-PAGE; WB.

(May be suitable for use in other assays to be determined by the end user.)

Predicted isoelectric point: 5.4 Accurate Molecular Mass: 340kDa Observe Molecular Mass: 70&56&52kDa Phenomenon explanation:

Fibrinogen is an abundant plasma protein (5-10uM) produced in the liver. The intact protein has a MW of 340kD. It is composed of 3 pairs of disulfide-bound polypeptide chains named Aalpha, Bbeta and gamma, with the weights of 70kDa, 56kDa, 52kDa, respectively.

[ USAGE ]

Reconstitute in PBS (PH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

[ STORAGE AND STABILITY ]

Storage: Avoid repeated freeze/thaw cycles.

Store at 2-8oC for one month.

Aliquot and store at -80oC for 12 months.

Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

[ IDENTIFICATION ]

Figure 1. SDS-PAGE