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Human Recombinant Adiponectin (ADP)
Instruction Manual!
| Product name: | Human Recombinant Adiponectin (ADP) |
| Source: | E. coli |
| Purity: | >98% |
| Buffer Formulation: | 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5%Trehalose and Proclin300. |
| Applications: | SDS-PAGE; WB; ELISA; IP; CoIP; Purification; Amine Reactive Labeling. |
| Storage: | Avoid repeated freeze/thaw cycles.Store at 2-8oC for one month.Aliquot and store at -80oC for 12 months. |
| UOM: | 50µg/100µg/200µg/1mg/5mg |
[ PROPERTIES ]
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Figure 2. SDS-PAGE |
Source: Prokaryotic expression.
Host: E. coli
Residues: Ala108~Asn244
Tags: N-terminal His-Tag
Tissue Specificity: Plasma.
Subcellular Location: Secreted.
Purity: >98%
Traits: Freeze-dried powder
Buffer formulation: 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5%Trehalose and Proclin300.
Original Concentration: 200ug/mL
Applications: SDS-PAGE; WB; ELISA; IP; CoIP; Purification; Amine Reactive Labeling.
(May be suitable for use in other assays to be determined by the end user.)
Predicted isoelectric point: 5.7
Predicted Molecular Mass: 22.5kDa
Accurate Molecular Mass: 21kDa as determined by SDS-PAGE reducing conditions.
[ USAGE ]
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
[ STORAGE AND STABILITY ]
Storage: Avoid repeated freeze/thaw cycles.
Store at 2-8oC for one month.
Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
[ SEQUENCE ]
[ IDENTIFICATION ]
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Figure 1. Gene Sequencing (Extract)
[ IMPORTANT NOTE ]
The kit is designed for research use only, we will not be responsible for any issue if the kit was used in clinical diagnostic or any other procedures.
