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Recombinant Mouse Vascular Endothelial Growth Factor A (VEGFA)
Instruction Manual!
| Product name: | Recombinant Mouse Vascular Endothelial Growth Factor A (VEGFA) |
| Source: | E. coli |
| Purity: | >98% |
| Buffer Formulation: | PBS, pH7.4, containing 1mM DTT, 5% trehalose, 0.01% sarcosyl and Proclin300 |
| Applications: | SDS-PAGE; WB; ELISA; IP; CoIP; Purification; Amine Reactive |
| Storage: | Avoid repeated freeze/thaw cycles.Store at 2-8oC for one month.Aliquot and store at -80oC for 12 months. |
| UOM: | 50µg/100µg/20µg/1mg/5mg |
[ PROPERTIES ]
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Source: Prokaryotic expression.
Host: E. coli
Residues: Ala27~Arg146
Tags: N-terminal His-Tag
Tissue Specificity: Spleen.
Subcellular Location: Secreted. Cell membrane; Peripheral membrane protein.
Purity: >98%
Traits: Freeze-dried powder
Buffer formulation: PBS, pH7.4, containing 1mM DTT, 5% trehalose, 0.01% sarcosyl and Proclin300.
Original Concentration: 200ug/mL
Applications: SDS-PAGE; WB; ELISA; IP; CoIP; Purification; Amine Reactive
Labeling. (May be suitable for use in other assays to be determined by the end user.)
Predicted isoelectric point: 6.5
Predicted Molecular Mass: 17.8kDa
Accurate Molecular Mass: 18kDa as determined by SDS-PAGE reducing conditions
[ USAGE ]
Reconstitute in PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex
[ STORAGE AND STABILITY ]
Storage: Avoid repeated freeze/thaw cycles.
Store at 2-8oC for one month.
Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
[ SEQUENCE ]
