Recombinant Peptidylglycine Alpha Amidating Monooxygenase(PAM)

[ PROPERTIES ]
Source: Prokaryotic expression.
Host: E. coli
Organism Species: Homo sapiens (Human)
Residues: Phe21~Cys288
Tags: N-terminal His-Tag
Tissue Specificity: Thyroid carcinoma, Heart, Thalamus, Lung, Kidney.
Subcellular Location: Membrane. Secreted.
Purity: >94%
Traits: Freeze-dried powder
Buffer formulation: 100mM NaHCO 3 , 500mM NaCl, pH8.3, containing 1mMEDTA, 1mM DTT, 0.01% sarcosyl, 5%Trehalose and Proclin300.
Original Concentration: 200ug/mL
Applications: SDS-PAGE; WB; ELISA; IP; CoIP; Purification; Amine ReactiveLabeling.
(May be suitable for use in other assays to be determined by the end user.)
Predicted isoelectric point: 8.8
Predicted Molecular Mass: 33.7kDa
Accurate Molecular Mass: 34kDa as determined by SDS-PAGE reducingconditions.
[ USAGE ]
Reconstitute in 100mM NaHCO 3 , 500mM NaCl (pH8.3) to a concentration of
0.1-1.0 mg/mL. Do not vortex.

[ STORAGE AND STABILITY ]
Storage: Avoid repeated freeze/thaw cycles.
Store at 2-8 o C for one month.
Aliquot and store at -80 o C for 12 months.
Stability Test: The thermal stability is described by the loss rate. The lossrate was determined by accelerated thermal degradation test, that is,incubate the protein at 37 o C for 48h, and no obvious degradation andprecipitation were observed. The loss rate is less than 5% within theexpiration date under appropriate storage condition.
[ SEQUENCE ]

[ IDENTIFICATION ]

Figure 1. SDS-PAGE