Recombinant Human Interleukin 6 (IL6)

[ PROPERTIES ]
Source: Prokaryotic expression.
Host: E. coli
Residues: Pro29~Met212
Tags: N-terminal GST Tag
Subcellular Location: Secreted
Purity: >95%
Traits: Freeze-dried powder
Buffer formulation: 20mM Tris, 150mM NaCl, pH8.0, containing 0.01% SKL,
5%Trehalose.
Original Concentration: 250ug/mL
Applications: Positive Control; Immunogen; SDS-PAGE; WB.
(May be suitable for use in other assays to be determined by the end user.)
Predicted isoelectric point: 6.0
Predicted Molecular Mass: 46.9kDa
Accurate Molecular Mass: 47kDa as determined by SDS-PAGE reducing
conditions.
[ USAGE ]
Reconstitute in 20mM Tris,150mM NaCl(pH8.0) to a concentration of 0.1-1.0 mg/mL.
Do not vortex.
[ STORAGE AND STABILITY ]
Storage: Avoid repeated freeze/thaw cycles.

Store at 2-8 o C for one month.
Aliquot and store at -80 o C for 12 months.
Stability Test: The thermal stability is described by the loss rate. The loss rate
was determined by accelerated thermal degradation test, that is, incubate the
protein at 37 o C for 48h, and no obvious degradation and precipitation were
observed. The loss rate is less than 5% within the expiration date under
appropriate storage condition.
[ SEQUENCE ]

[ IDENTIFICATION ]

Figure . Gene Sequencing (Extract)

Figure. SDS-PAGE