Recombinant Human Complement Component 3 (C3)

Instruction Manual!

Product name:	Recombinant Human Complement Component 3 (C3)
Source:	E. coli
Purity:	> 97%
Buffer Formulation:	PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
Applications:	Positive Control; Immunogen; SDS-PAGE; WB. (May be suitable for use in other assays to be determined by the end user.)
Storage:	Avoid repeated freeze/thaw cycles,Store at 2-8oC for one month,Aliquot and store at -80oC for 12 months.
UOM:	50µg/200µg/1mg/5mg

Recombinant Human Complement Component 3 (C3)

[ PROPERTIES ]

SDS-PAGE

Source: Prokaryotic expression

Host: E.coli

Residues: Leu1000~Glu1326

Tags: Two N-terminal Tags, His-tag and SUMO-tag

Subcellular Location: Secreted

Purity: > 97%

Traits: Freeze-dried powder

Buffer formulation: PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.

Original Concentration: 200µg/mL

Applications: Positive Control; Immunogen; SDS-PAGE; WB. (May be suitable for use in other assays to be determined by the end user.)

Predicted isoelectric point: 6.0

Predicted Molecular Mass: 43.4kDa

Accurate Molecular Mass: 43kDa as determined by SDS-PAGE reducing conditions.

[ USAGE ]

Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

[ STORAGE AND STABILITY ]

Storage: Avoid repeated freeze/thaw cycles.

Store at 2-8oC for one month.

Aliquot and store at -80oC for 12 months.

Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

[ SEQUENCE ]

[ IDENTIFICATION ]

Figure. Gene Sequencing (Extract)