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Recombinant Collagen Type XI Alpha 1 (COL11a1)

Recombinant Collagen Type XI Alpha 1 (COL11a1) Recombinant Collagen Type XI Alpha 1 (COL11a1)

Instruction Manual!

Product name: Recombinant Collagen Type XI Alpha 1 (COL11a1)
Source:E. coli
Purity:> 95%
Buffer Formulation:20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
Applications:Positive Control; Immunogen; SDS-PAGE; WB.
Storage:Avoid repeated freeze/thaw cycles.
UOM:50µg/100µg/200µg/1mg/5mg

Recombinant Collagen Type XI Alpha 1 (COL11a1)

[ PROPERTIES ]

Source: Prokaryotic expression

Host: E.coli

Residues: Asp1564~Gly1806

Tags: N-terminal His Tag

Subcellular  Location: Secreted, Extracellular matrix

Purity: > 95%

Traits: Freeze-dried powder
Buffer formulation: 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT,0.01% SKL, 5% Trehalose .

Original Concentration: 200µg/mL
Applications: Positive Control; Immunogen; SDS-PAGE; WB.
(May be suitable for use in other assays to be determined by the end user.)

Predicted isoelectric point: 4.7
Predicted Molecular Mass: 31.2kDa
Accurate Molecular Mass: 32kDa as determined by SDS-PAGE reducing conditions.

[ USAGE ]
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do notvortex.

[ STORAGE AND STABILITY ]
Storage: Avoid repeated freeze/thaw cycles.
 Store at 2-8ºC for one month.
 Aliquot and store at -80ºC for 12 months.

Stability Test: The thermal stability is described by the loss rate. The loss rate was determinedby accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and noobvious degradation and precipitation were observed. The loss rate is less than 5% within theexpiration date under appropriate storage condition.

[ SEQUENCE ]

[ IDENTIFICATION ]

[ IMPORTANT NOTE ]
The kit is designed for research use only, we will not be responsible for any issue if the kit wasused in clinical diagnostic or any other procedures.

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