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Recombinant Human Glycoprotein 2, Zymogen Granule Membrane (GP2)/ZAP75
Instruction Manual!
| Product name: | Recombinant Human Glycoprotein 2, Zymogen Granule Membrane (GP2)/ZAP75 |
| Source: | E. coli |
| Purity: | >95% |
| Buffer Formulation: | Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl. |
| Applications: | SDS-PAGE; WB; ELISA; IP |
| Storage: | Avoid repeated freeze/thaw cycles. |
| UOM: | 50µg/100µg/200µg/1mg/5mg |
Recombinant Human Glycoprotein 2, Zymogen Granule Membrane (GP2)/ZAP75
[ PROPERTIES ]
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Figure 2. SDS-PAGE |
Residues: Phe307~Asn512 (Accession # P55259), with two N-terminal Tags, His-tag and T7-tag.
Host: E. coli
Subcellular Location: Cell membrane; Lipid-anchorGPI-anchor. Secreted.
Purity: >95%
Endotoxin Level: <1.0EU per 1μg (determined by the LAL method).
Formulation: Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
Predicted isoelectric point: 5.6
Predicted Molecular Mass: 26.9kDa Applications: SDS-PAGE; WB; ELISA; IP.
(May be suitable for use in other assays to be determined by the end user.)
[ USAGE ]
Reconstitute in sterile PBS, pH7.2-pH7.4.
[ STORAGE AND STABILITY ]
Storage: Avoid repeated freeze/thaw cycles.
Store at 2-8oC for one month.
Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
[ SEQUENCE ]
The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.
MG SSH HHH HH SSGLVPR GSH MAS MTG GQ Q M GRG SEF- FIIR DTI LN INFQ C AYPLDMKVSL QAALQPIVSS LNVSVDGNGE FIVRMALFQD QNYTNPYEGD AVELSVESVL YVGAILEQGD TSRFNLVLRN CYATPTEDKA DLVKYFIIRN SCSNQRDSTI HVEENGQSSE SRFSVQMFMF AGHYDLVFLH CEIHLCDSLN EQCQPSCSRS QVRSEVPAID LARVLDLGPI TRRGAQSPGV MN
[ IMPORTANT NOTE ]
The kit is designed for research use only, we will not be responsible for any issue if the kit was used in clinical diagnostic or any other procedures.
