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Recombinant Mouse Hemicentin 1 (HMCN1)
Instruction Manual!
| Product name: | Recombinant Mouse Hemicentin 1 (HMCN1) |
| Source: | E. coli |
| Purity: | > 90% |
| Buffer Formulation: | 20mM Tris, 150mM NaCl, pH8.0, containing 0.01% SKL, 5% Trehalose. |
| Applications: | Positive Control; Immunogen; SDS-PAGE; WB. |
| Storage: | Avoid repeated freeze/thaw cycles. |
| UOM: | 50µg/200µg/1mg/5mg |
[ PROPERTIES ]
Source: Prokaryotic expression
Host: E.coli
Residues: Gly22~Val153
Tags: N-terminal His and GST Tag
Subcellular Location: n/a
Purity: > 90%
Traits: Freeze-dried powder
Buffer formulation: 20mM Tris, 150mM NaCl, pH8.0, containing 0.01% SKL, 5% Trehalose.
Original Concentration: 200µg/mL
Applications: Positive Control; Immunogen; SDS-PAGE; WB.
(May be suitable for use in other assays to be determined by the end user.)
Predicted isoelectric point: 4.9
Predicted Molecular Mass: 18.4kDa
Accurate Molecular Mass: 45kDa as determined by SDS-PAGE reducing conditions.
Phenomenon explanation:
The possible reasons that the actual band size differs from the predicted are as follows:
1.Splice variants: Alternative splicing may create different sized proteins from the same gene.
2. Relative charge: The composition of amino acids may affects the charge of the protein.
3. Post-translational modification: Phosphorylation, glycosylation, methylation etc.
4. Post-translation cleavage: Many proteins are synthesized as pro-proteins, and then cleaved to give the active form.
5. Polymerization of the target protein: Dimerization, multimerization etc.
[ USAGE ]
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
[ STORAGE AND STABILITY ]
Storage: Avoid repeated freeze/thaw cycles.
Store at 2-8ºC for one month.
Aliquot and store at -80ºC for 12 months.
Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
[ SEQUENCE ]
[ IDENTIFICATION ]
Figure. Gene Sequencing (Extract)
Figure. SDS-PAGE
