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Recombinant 17-Beta-Hydroxysteroid Dehydrogenase Type 10 (HSD17b10)
Instruction Manual!
| Product name: | Recombinant 17-Beta-Hydroxysteroid Dehydrogenase Type 10 (HSD17b10) |
| Source: | E. coli |
| Purity: | >95% |
| Buffer Formulation: | Supplied as lyophilized form in PBS,pH7.4, containing 5% trehalose, 0.01% sarcosyl. |
| Applications: | SDS-PAGE; WB; ELISA; IP. |
| Storage: | Avoid repeated freeze/thaw cycles. |
| UOM: | 50µg/200µg/1mg/5mg |
[ PROPERTIES ]
Residues: Ala4~Ala202
Tags: Two N-terminal Tags, His-tag and T7-tag
Accession: O08756
Host: E. coli
Subcellular Location: Mitochondrion.
Purity: >95%
Endotoxin Level: <1.0EU per 1μg (determined by the LAL method).
Formulation: Supplied as lyophilized form in PBS,pH7.4, containing 5% trehalose, 0.01% sarcosyl.
Predicted isoelectric point: 8.9
Predicted Molecular Mass: 24.1kDa
Applications: SDS-PAGE; WB; ELISA; IP.
(May be suitable for use in other assays to be determined by the end user.)
[ USAGE ]
Reconstitute in sterile PBS, pH7.2-pH7.4.
[ STORAGE AND STABILITY ]
Storage: Avoid repeated freeze/thaw cycles.
Store at 2-8oC for one month.
Aliquot and store at -80oC for 12 months.
Stability Test:
The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37oC for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.
[ SEQUENCE ]
The sequence of the target protein is listed below.
AVRSVKG LVAVVTGGAS GPWLATAKRL VGQGATAVLL DVPDSEGESQ AKKLGESCIF APANVTSEKE IQAALTLAKE KFGRIDVAVN CAGIAVAIKT YHQKKNKIHT LEDFQRVINV NLIGTFNVIR LVAGEMGQNE PDQGGQRGVI INTASVAAFE GQVGQAAYSA SKGGIDGMTL PIARDLAPTG IRVVTIAPGL FA
[ IDENTIFICATION ]
[ IMPORTANT NOTE ]
The kit is designed for research use only, we will not be responsible for any issue if the kit was used in clinical diagnostic or any other procedures.
