Recombinant Mouse Neurocan (NCAN)

Instruction Manual!

Product name:	Recombinant Mouse Neurocan (NCAN)
Source:	E. coli
Purity:	>95%
Buffer Formulation:	Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
Applications:	SDS-PAGE; WB; ELISA; IP
Storage:	Avoid repeated freeze/thaw cycles.
UOM:	50µg/100µg/200µg/1mg/5mg

[ PROPERTIES ]

Residues: Pro960~Lys1225

Tags: Two N-terminal Tags, His-tag and T7-tag

Accession: P55066

Host: E. coli

Subcellular Location: Secreted.

Purity: >95%

Endotoxin Level: <1.0EU per

1μg (determined by the LAL

method).

Formulation: Supplied as lyophilized form in PBS,

pH7.4, containing 5% trehalose, 0.01% sarcosyl.

Predicted isoelectric point: 5.7

Predicted Molecular Mass: 33.8kDa

Accurate Molecular Mass: 38kDa as determined by SDS-PAGE reducing

conditions.

Applications: SDS-PAGE; WB; ELISA; IP.

(May be suitable for use in other assays to be determined by the end user.)

Note: The possible reasons that the actual band size differs from the predicted are

as follows:

1. Splice variants: Alternative splicing may create different sized proteins from the same gene.

2. Relative charge: The composition of amino acids may affects the charge of theprotein.

3. Post-translational modification: Phosphorylation, glycosylation, methylation etc.

4. Post-translation cleavage: Many proteins are synthesized as pro-proteins, and then

cleaved to give the active form.

5. Polymerization of the target protein: Dimerization, multimerization etc.

[ USAGE ]

Reconstitute in sterile PBS, pH7.2-pH7.4.

[ STORAGE AND STABILITY ]

Storage: Avoid repeated freeze/thaw cycles.

Store at 2-8oC for one month.

Aliquot and store at -80oC for 12 months.

Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products

Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.

[ SEQUENCES ]

The sequence of the target protein is listed below.

P TDPCENNPCL HGGTCHTNGT VYGCSCDQGY AGENCEIDID

DCLCSPCENG GT C I D EV N G F IC L C LPSY G G SLC EK D TE GC DR G

WH KF Q GH CY RY FA H R R A WE D AE R D C R R RA G H LTS V H S PE EH K

F IN SF GH E N S WI GLN DRT VE R D FQ W TDNTGLQYEN

WREKQPDNFF AGGEDCVVMV AHESGRWNDV PCNYNLPYVC

KKGTVLCGPP PAVENASLVG VRKIKYNVHA TVRYQCDEGF SQHRVATIRC

RNNGKWDRPQ IMCIK