ELISA kit Validity Stability Test
Product Name: Human Growth Differentiation Factor 15 (GDF15) ELISA Kit
1. OD Value
OD Values contrast data was as below(Under right storage conditions):
Normal period of validity OD Values: 3.251, 2.845, 2.126, 1.859, 1.463, 1.022, 0.710, 0.08
After 12 months period of validity OD Values:3.1.7, 2.643, 2.125, 1.796, 1.453, 0.876, 0.432, 0.07
2. Recovery
Matrices listed below were spiked with certain level of recombinant human GDF 15 and the recovery rates were calculated by comparing the measured value to the expected amount of GDF 15 in samples.
Normal and after 12 months Normal period of validity ELISA Kit Recovery results
3. Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of human GDF 15 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Normal and after 12 months Normal period of validity ELISA Kit Linearity results
4. Precision
CV(%) = SD/meanX100
Intra-assay Precision (Precision within one batch): 3 samples with low, middle and high level human GDF15 were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between batches): 3 samples with low, middle and high level human GDF15 were tested on 3 different plates, 8 replicates in each plate.
(Intra-assay Precision:CV<10%; Inter-assay Precision:CV<11%)
Normal and after 12 months Normal period of validity ELISA Kit Precision results
Intra-assay precision
Inter-assay precision
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1. OD Value
OD Values contrast data was as below(Under right storage conditions):
Normal period of validity OD Values: 3.251, 2.845, 2.126, 1.859, 1.463, 1.022, 0.710, 0.08
After 12 months period of validity OD Values:3.1.7, 2.643, 2.125, 1.796, 1.453, 0.876, 0.432, 0.07
2. Recovery
Matrices listed below were spiked with certain level of recombinant human GDF 15 and the recovery rates were calculated by comparing the measured value to the expected amount of GDF 15 in samples.
Normal and after 12 months Normal period of validity ELISA Kit Recovery results
| Martrix | Conditions | Recovery range(%) | Average(%) |
| Serum(n=6) | normal | 88-106 | 95 |
| After 12 month | 80-100 | 87 | |
| EDTA plasma(n=6) | normal | 88-106 | 97 |
| After 12 month | 78-98 | 87 |
3. Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of human GDF 15 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Normal and after 12 months Normal period of validity ELISA Kit Linearity results
| Sample | Conditions | 1:2 | 1:4 | 1:8 |
| Serum(n=6) | normal | 81-92% | 85-90% | 89-105% |
| After 12month | 80-88% | 82-87% | 87-101% | |
| EDTA plasma(n=6) | normal | 93-104% | 81-97% | 91-96% |
| After 12month | 82-95% | 75-82% | 74-90% |
4. Precision
CV(%) = SD/meanX100
Intra-assay Precision (Precision within one batch): 3 samples with low, middle and high level human GDF15 were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between batches): 3 samples with low, middle and high level human GDF15 were tested on 3 different plates, 8 replicates in each plate.
(Intra-assay Precision:CV<10%; Inter-assay Precision:CV<11%)
Normal and after 12 months Normal period of validity ELISA Kit Precision results
Intra-assay precision
| Conditions | normal | After 12month | normal | After 12month | normal | After 12month |
| sample | 1 | 1 | 2 | 2 | 3 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean(ng/ml) | 16.68 | 14.22 | 70.25 | 62.36 | 369.75 | 327.88 |
| SD | 1.08 | 1.25 | 4.21 | 5.31 | 24.69 | 32.87 |
| CV(%) | 6.5 | 8.8 | 6.0 | 8.5 | 6.6 | 10 |
Inter-assay precision
| Conditions | normal | After 12month | normal | After 12month | normal | After 12month |
| Sample | 1 | 1 | 2 | 2 | 3 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean(ng/ml) | 18.72 | 13.68 | 65.98 | 61.25 | 387.22 | 425.26 |
| SD | 1.45 | 1.52 | 4.83 | 5.18 | 28.49 | 36.87 |
| CV（%） | 7.7 | 11.1 | 7.3 | 8.5 | 7.3 | 8.7 |
You can inquiry our elisa kits as
