Active Interleukin 4 (IL4)

Instruction Manual!

Product name:	Active Interleukin 4 (IL4)
Source:	Natural Extract
Purity:	>95%
Buffer Formulation:	20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5% trehalose, and Proclin300.
Applications:	: Cell culture; Activity Assays; In vivo assays. (May be suitable for use in other assays to be determined by the end user.)
Storage:	Avoid repeated freeze/thaw cycles. Store at 2-8
UOM:	50ug/100ug/200ug/1mg

roduct No.ICA077Hu01
Organism SpeciesHomo sapiens (Human)Same name, Different species.
Buffer Formulation20mM Tris, 150mM NaCl,pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
TraitsFreeze-dried powder
Purity> 95%
Isoelectric Point9.3
ApplicationsCell culture; Activity Assays; In vivo assays.
DownloadInstruction Manual
UOM10ug50ug200ug1mg1g
FOBUS$ 631

ACTIVITY TEST

As reported, IL-4 will induce the differentiation of THP-1 cells into dendritic cells and macrophages in vitro. THP-1 cells were cultured in RPMI-1640 and stimulated with 2ng/mL IL-4, after 10 days of stimulation, partial cells became larger, grew synapses, and had vesicular bodies intracellular, which showed a morphological characteristics of dendritic cells and macrophages.

USAGE

Reconstitute in 20mM Tris, 150mM NaCl (PH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

STORAGE

Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

STABILITY

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

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