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A Disintegrin And Metalloprotease 1 Recombinant Protein

A Disintegrin And Metalloprotease 1 Recombinant Protein A Disintegrin And Metalloprotease 1 Recombinant Protein

Instruction Manual!

Product name: A Disintegrin And Metalloprotease 1 Recombinant Protein
Source:
Purity:>95%
Buffer Formulation:Reconstitute in sterile PBS, pH7.2-pH7.4.
Applications:SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
Storage:Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.
UOM:10ug/50ug/100ug/200ug/1mg

Product Name

  

A Disintegrin And Metalloprotease 1 (ADAM1), Recombinant Protein

 Also Known As

  

Recombinant A Disintegrin And Metalloprotease 1 (ADAM1)

 Product Gene Name

  

ADAM1 recombinant protein

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 Research Use Only

  

For Research Use Only. Not for use in diagnostic procedures.

 

 

 Sequence

  

The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.
SHPCCEPT CTLKVGAQCS EGLCCYKCTF KKKGTLCRPA EDVCDLPEYC NGITGECPAN SYMQDGTQCD RIYYCSGGLC KNPDKQCARI YGYPARSAPE ECYISVNTKA NRFGNCGHPT SANLKYEACS NEDIFCGKLV CTDVRYLPQV KPLHSLLQIP YGDDWCWSMD AYNVTDIPDY GDVQGGTYCA PKKVCMESIC TGHATLQYDC HPQEMCHGNG VCNNFKHCHC DAGFSPPDCS SGGNGGSVDS GPVGKPADRN LSLFGVG

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 Host

  

Host: E Coli
Source: Prokaryotic expression

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 Purity/Purification

  

>95%

 Form/Format

  

Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.

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 Predicted Molecular Mass

  

32.4kDa

 Accurate Molecular Mass (KD)

  

37kDa

 Endotoxin

  

<1.0EU per 1ug (determined by the LAL method)

 Expression System

  

Prokaryotic expression

 Tag

  

N-terminal His-Tag, T7 tag

 Organism Species

  

Rattus norvegicus (Rat)

 Fragment

  

Ser463~Gly727

 Usage

  

Reconstitute in sterile PBS, pH7.2-pH7.4.

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 Preparation and Storage

  

Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.
Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. The loss of this protein is less than 5% within the expiration date under appropriate storage condition.

 Other Notes

  

Small volumes of ADAM1 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

 SDS-Page of ADAM1 recombinant protein    ADAM1 recombinant protein SDS-Page image

 

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