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Recombinant Brain Derived Neurotrophic Factor (BDNF)
Instruction Manual!
| Product name: | Recombinant Brain Derived Neurotrophic Factor (BDNF) |
| Source: | Prokaryotic expression |
| Purity: | > 95% |
| Buffer Formulation: | Buffer Formulation20mM Tris, 150mM NaCl,pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300. |
| Applications: | SDS-PAGE; WB; ELISA; IP. |
| Storage: | Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months |
| UOM: | 50ug/100ug/200ug/1mg/1g |
- Product No.ICA011Mi01
- Organism SpeciesHomo sapiens (Human), Mus musculus (Mouse), Rattus norvegicus (Rat), Oryctolagus cuniculus (Rabbit), Sus scrofa; Porcine (Pig), Capra hircus; Caprine (Goat), Equus caballus; Equine (Horse)Same name, Different species.
- SourceProkaryotic expression
- HostE.coli
- Endotoxin Level<1.0EU per 1µg (determined by the LAL method)
- Subcellular LocationSecreted
- Predicted Molecular Mass27.4kDa
- Accurate Molecular Mass32kDa(Analysis of differences refer to the manual)
- Residues & TagsPro20~Arg252 with N-terminal His Tag
- Buffer Formulation20mM Tris, 150mM NaCl,pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
- TraitsFreeze-dried powder
- Purity> 95%
- Isoelectric Point8.8
- ApplicationsSDS-PAGE; WB; ELISA; IP; CoIP; Purification; Amine Reactive Labeling.
- Downloadn/a
- UOM10ug50ug200ug1mg1g
- FOB
SEQUENCE
USAGE
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
STORAGE
Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
STABILITY
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
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