| ELISA kit |
| Clinical diagnosis |
| Trfia |
| ANTIBODIES |
| CLIA kits |
| Proteins |
| New Products |
| > Human |
| > Canine |
| > Bovine |
| > Equine |
| > Fish |
| > Mouse |
| > Rat |
| > Chicken |
| > Porcine |
| > Rabbit |
| > Goat |
| > Simian |
| > General |
| > Cavia |
| > Caprine |
| > Sheep |
| > Cattle |
Active Interleukin 6 (IL6)
Instruction Manual!
| Product name: | Active Interleukin 6 (IL6) |
| Source: | |
| Purity: | > 95% |
| Buffer Formulation: | PBS, pH7.6, containing 5% trehalose. |
| Applications: | Cell culture; Activity Assays; In vivo assays. |
| Storage: | Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months. |
| UOM: | 50ug/100ug/200ug/1mg/1g |
-
- Product No.ICA079Ra61
- Organism SpeciesRattus norvegicus (Rat)Same name, Different species.
- TraitsFreeze-dried powder
- Purity> 95%
- Isoelectric Point7.8
- ApplicationsCell culture; Activity Assays; In vivo assays.
- DownloadInstruction Manual
- UOM10ug50ug200ug1mg1g
- FOB
ACTIVITY TEST
Interleukin-6 (IL-6), a pro-inflammatory cytokine and an anti-inflammatory myokine, plays important roles in the acute phase reaction, inflammation, hematopoiesis, bone metabolism, and cancer progression. It has been reported that IL-6 significantly increased the MMP-10 protein lever in the human lung cancer A549 cells through the JAK/STAT signaling pathway. Briefly, A549 cells were seeded into 6-well cell culture clusters and allowed to grow to 50-70% confluence, then different concentrations of IL-6 was added. After incubated for 24h, the protein levels of MMP-10 in the cell supernatant were determined by Western blot.
Result: MMP-10 protein lever significantly increased in A549 cells due to the stimulation of IL-6, the data was shown in Figure 1.USAGE
Reconstitute in PBS (pH7.6) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
STORAGE
Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
STABILITY
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
GIVEAWAYS
ELISA/CLIA Experiment Service Offer
PCR Mix System OfferINCREMENT SERVICES
