Recombinant Cluster Of Differentiation 19 (CD19)

[ PROPERTIES ]

Residues: Arg19~Gly287

Tags: Two N-terminal Tags, His-tag and T7-tag

Accession: P25918

Host: E. coli

Subcellular Location: Membrane. Single-pass type I membrane protein.

Purity: >90%

Endotoxin Level: <1.0EU per 1μg (determined by the LAL method).

Formulation: Supplied as lyophilized form in 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5% trehalose, and preservative.

Predicted isoelectric point: 8.6

Predicted Molecular Mass: 33.3kDa

Applications: SDS-PAGE; WB; ELISA; IP.

(May be suitable for use in other assays to be determined by the end user.)

[ USAGE ]

Reconstitute in sterile ddH2O.

[ STORAGE AND STABILITY ]

Storage: Avoid repeated freeze/thaw cycles.Store at 2-8^oC for one month.

Aliquot and store at -80^oC for 12 months.

Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate wasdetermined by accelerated thermal degradation test, that is, incubate the protein at 37^oC for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.

[ SEQUENCES ]

The sequence of the target protein is listed below.

RP QK SL LV E V E E GG N V V LP C L P DSS P V S S E K L AW Y R G N Q S T P FL E LS P G S P G

LGLHVGSLGI LLVIVNVSDH MGGFYLCQKR PPFKDIWQPA WTVNVEDSGE MFRWNASDVR

DLDCDLRNRS SGSHRSTSGS QLYVWAKDHP KVWGTKPVCA PRGSSLNQSL INQDLTVAPG

STLWLSCGVP PVPVAKGSIS WTHVHPRRPN VSLLSLSLGG EHPVREMWVW GSLLLLPQAT

ALDEGTYYCL RGNLTIERHV KVIARSAVWL WLLRTGG