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A Disintegrin And Metalloprotease 17 (ADAM17), Recombinant Protein
Instruction Manual!
| Product name: | A Disintegrin And Metalloprotease 17 (ADAM17), Recombinant Protein |
| Source: | Rattus norvegicus (Rat) |
| Purity: | >95% |
| Buffer Formulation: | Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl. |
| Applications: | SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP) |
| Storage: | Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months. |
| UOM: | 10ug/50ug/100ug/200ug/1mg |
Product Name :A Disintegrin And Metalloprotease 17 (ADAM17), Recombinant Protein
Also Known As : Recombinant A Disintegrin And Metalloprotease 17 (ADAM17)
Product Gene Name : ADAM17 recombinant protein [Similar Products]
Research Use Only For Research Use Only. Not for use in diagnostic procedures.
| Sequence |
The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below. LKNTCKLLV V ADHRFYKYMG RGEESTTTNY LIELIDRVDD IYRNTSWDNA GFKGYGVQIE QIRILKSPQE VKPGERHFNM AKSFPNEEKD AWDVKMLLEQ FSLDIAEEAS KVCLAHLFTY QDFDMGTLGL AYVGSPRANS HGGVCPKAYY NPGVKKNIYL NSGLTSTKNY GKTILTKEAD LVTTHELGHN FGAEHDPDGL AECAPNEDQG GKYVMYPIAV SGDHENNKMF S |
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| Host |
Host: E Coli Source: Prokaryotic expression |
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| Purity/Purification | >95% | ||
| Form/Format | Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl. | ||
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| Predicted Molecular Mass | 29.6kDa | ||
| Accurate Molecular Mass (KD) | 30kDa | ||
| Endotoxin | <1.0EU per 1ug (determined by the LAL method) | ||
| Expression System | Prokaryotic expression | ||
| Tag | N-terminal His-Tag | ||
| Organism Species | Rattus norvegicus (Rat) | ||
| Fragment | Leu221~Ser451 | ||
| Usage | Reconstitute in sterile PBS, pH7.2-pH7.4. | ||
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| Preparation and Storage |
Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months. Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. The loss of this protein is less than 5% within the expiration date under appropriate storage condition. |
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| Other Notes | Small volumes of ADAM17 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply. | ||
| Applications Tested/Suitable for ADAM17 recombinant protein |
SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP) |
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| SDS-Page of ADAM17 recombinant protein |
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