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Recombinant A Disintegrin And Metalloprotease 1 (ADAM1)

Recombinant A Disintegrin And Metalloprotease 1 (ADAM1) Recombinant A Disintegrin And Metalloprotease 1 (ADAM1)

Instruction Manual!

Product name: Recombinant A Disintegrin And Metalloprotease 1 (ADAM1)
Source:Prokaryotic expression
Purity:>95%
Buffer Formulation:Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
Applications:SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
Storage:Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.
UOM:10ug/50ug/100ug/200ug/1mg
 Product Name :  A Disintegrin And Metalloprotease 1 (ADAM1), Recombinant Protein
 Also Known As:    Recombinant A Disintegrin And Metalloprotease 1 (ADAM1)
 Product Gene Name:    ADAM1 recombinant protein [Similar Products]
 Research Use Only   For Research Use Only. Not for use in diagnostic procedures.
Sequence    The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.
SHPCCEPT CTLKVGAQCS EGLCCYKCTF KKKGTLCRPA EDVCDLPEYC NGITGECPAN SYMQDGTQCD RIYYCSGGLC KNPDKQCARI YGYPARSAPE ECYISVNTKA NRFGNCGHPT SANLKYEACS NEDIFCGKLV CTDVRYLPQV KPLHSLLQIP YGDDWCWSMD AYNVTDIPDY GDVQGGTYCA PKKVCMESIC TGHATLQYDC HPQEMCHGNG VCNNFKHCHC DAGFSPPDCS SGGNGGSVDS GPVGKPADRN LSLFGVG
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 Host    Host: E Coli
Source: Prokaryotic expression
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 Purity/Purification    >95%
 Form/Format    Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
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 Predicted Molecular Mass    32.4kDa
 Accurate Molecular Mass (KD)    37kDa
 Endotoxin    <1.0EU per 1ug (determined by the LAL method)
 Expression System    Prokaryotic expression
 Tag    N-terminal His-Tag, T7 tag
 Organism Species    Rattus norvegicus (Rat)
 Fragment    Ser463~Gly727
 Usage    Reconstitute in sterile PBS, pH7.2-pH7.4.
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 Preparation and Storage    Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.
Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
 Other Notes    Small volumes of ADAM1 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
 Applications Tested/Suitable forADAM1 recombinant protein   

SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)

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 SDS-Page of ADAM1 recombinant protein    ADAM1 recombinant protein SDS-Page image

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