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Recombinant A Disintegrin And Metalloprotease 10 (ADAM10)

Recombinant A Disintegrin And Metalloprotease 10 (ADAM10) Recombinant A Disintegrin And Metalloprotease 10 (ADAM10)

Instruction Manual!

Product name: Recombinant A Disintegrin And Metalloprotease 10 (ADAM10)
Source:Homo sapiens (Human)
Purity:> 95%
Buffer Formulation:Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
Applications:SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
Storage:Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.
UOM:10ug/50ug/100ug/200ug/1mg
 Product Name   A Disintegrin And Metalloprotease 10 (ADAM10), Recombinant Protein
 Also Known As   Recombinant A Disintegrin And Metalloprotease 10 (ADAM10)
 Product Gene Name   ADAM10 recombinant protein [Similar Products]
 Research Use Only   For Research Use Only. Not for use in diagnostic procedures.
Sequence    The target protein is fused with N-terminal His-Tag, its sequence is listed below.
MGHHHHHHSG SEF-R ATSGDKLNNN KFSLCSIRNI SQVLEKKRNN CFVESGQPIC GNGMVEQGEE CDCGYSDQCK DECCFDANQP EGRKCKLKPG KQCSPSQGPC CTAQCAFKSK SEKCRDDSDC AREGICNGFT ALCPASDPKP NFTDCNRHTQ VCINGQCAGS ICEKYGLEEC TCASSDGKDD KELCHVCCMK KMDPSTCAST GSVQWSRHFS GRTITLQPGS PCNDFRGYCD V
 Chromosome Location    Chromosome: 15; NC_000015.9 (58887403..59042177, complement). Location: 15q22
 OMIM    602192
 3D Structure    ModBase 3D Structure for O14672
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 Host    E Coli
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 Purity/Purification    > 95%
 Form/Format    Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
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 Organism    Homo sapiens (Human)
 Expression System    Prokaryotic expression
 Residues    Arg420~Val641 (Accession # O14672) with N-terminal His-Tag
 Subcellular Location    Cell membrane; Single-pass type I membrane protein. Endomembrane system
 Endotoxin Level    <1.0EU per 1ug (determined by the LAL method)
 Predicted Isoelectric Point    6.5
 Reconstitution    Reconstitute in sterile PBS, pH7.2-pH7.4.
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 Preparation and Storage    Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.
Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.
 Other Notes    Small volumes of ADAM10 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Product Description specifically for ADAM10 recombinant protein

   About the Marker: Effective Size Range: 10kDa to 70kDa.
Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and70kDa.
Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at doubleintensity to make location and size approximation of proteins of interestquick and easy.
Ready-to-use: No need to heat, dilute or add reducing agents before use.
 Applications Tested/Suitable forADAM10 recombinant protein   

SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)

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 SDS-PAGE of ADAM10 recombinant protein    ADAM10 recombinant protein SDS-PAGE image

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