Recombinant A Disintegrin And Metalloproteinase With Thrombospondin 1 (ADAMTS1)

Instruction Manual!

Product name:	Recombinant A Disintegrin And Metalloproteinase With Thrombospondin 1 (ADAMTS1)
Source:	Prokaryotic expression
Purity:	> 95%
Buffer Formulation:	Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
Applications:	SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
Storage:	Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.
UOM:	10ug/50ug/100ug/200ug/1mg

Product Name A Disintegrin And Metalloproteinase With Thrombospondin 1 (ADAMTS1), Recombinant Protein
Also Known As Recombinant A Disintegrin And Metalloproteinase With Thrombospondin 1 (ADAMTS1)
Product Gene Name ADAMTS1 recombinant protein [Similar Products]
Research Use Only For Research Use Only. Not for use in diagnostic procedures.

Sequence    The target protein is fused with N-terminal His-Tag, its sequence is listed below.
MGHHHHHHSG SEF-H GSWGPWGPWG DCSRTCGGGV QYTMRECDNP VPKNGGKYCE GKRVRYRSCN IEDCPDNNGK TFREEQCEAH NEFSKASFGN EPTVEWTPKY AGVSPKDRCK LTCEAKGIGY FFVLQPKVVD GTPCSPDSTS VCVQGQCVKA GCDRIIDSKK KFDKCGVCGG NGSTCKKMSG IVTSTRPGYH DIVTIPAGAT NIEVKHRNQR GSRNNGSFLA IRAADGTYIL NGNFTLSTLE QDLTYKGTVL RYSGSSAALE RIRSFSPLKE PLTIQVLMVG HALRPKIKFT YFMKKKTESF NAIPTFSEWV IEEWGECSKT CGSGWQRRVV QCRDINGHPA SECAKEVKPA STRPCADLPC P

Chromosome Location    Chromosome: 16; NC_000082.6 (85793827..85803115, complement). Location: 16 C3-C5; 16 48.2 cM

3D Structure    ModBase 3D Structure for P97857

Host    E Coli

Purity/Purification    > 95%

Form/Format    Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.

Organism    Mus musculus (Mouse)

Expression System    Prokaryotic expression

Residues    His560~Pro911 (Accession # P97857) with N-terminal His-Tag

Endotoxin Level    <1.0EU per 1ug (determined by the LAL method)

Reconstitution    Reconstitute in sterile PBS, pH7.2-pH7.4.

Preparation and Storage    Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.
Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

Other Notes    Small volumes of ADAMTS1 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Product Description specifically for ADAMTS1 recombinant protein
   About the Marker: Effective Size Range: 10kDa to 70kDa.
Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and70kDa.
Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at doubleintensity to make location and size approximation of proteins of interestquick and easy.
Ready-to-use: No need to heat, dilute or add reducing agents before use.

Applications Tested/Suitable forADAMTS1 recombinant protein
SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)

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NCBI/Uniprot data below describe general gene information for ADAMTS1. It may not necessarily be applicable to this product.

NCBI GI #    157951646

NCBI GeneID    11504

NCBI Accession #    NP_033751.3 [Other Products]

NCBI GenBank Nucleotide #    NM_009621.4 [Other Products]

UniProt Primary Accession #    P97857 [Other Products]

UniProt Secondary Accession #    O54768; E9QMN9 [Other Products]

UniProt Related Accession #    P97857 [Other Products]

Molecular Weight    40.2kDa

Sequence		The target protein is fused with N-terminal His-Tag, its sequence is listed below. MGHHHHHHSG SEF-H GSWGPWGPWG DCSRTCGGGV QYTMRECDNP VPKNGGKYCE GKRVRYRSCN IEDCPDNNGK TFREEQCEAH NEFSKASFGN EPTVEWTPKY AGVSPKDRCK LTCEAKGIGY FFVLQPKVVD GTPCSPDSTS VCVQGQCVKA GCDRIIDSKK KFDKCGVCGG NGSTCKKMSG IVTSTRPGYH DIVTIPAGAT NIEVKHRNQR GSRNNGSFLA IRAADGTYIL NGNFTLSTLE QDLTYKGTVL RYSGSSAALE RIRSFSPLKE PLTIQVLMVG HALRPKIKFT YFMKKKTESF NAIPTFSEWV IEEWGECSKT CGSGWQRRVV QCRDINGHPA SECAKEVKPA STRPCADLPC P
Chromosome Location		Chromosome: 16; NC_000082.6 (85793827..85803115, complement). Location: 16 C3-C5; 16 48.2 cM
3D Structure		ModBase 3D Structure for P97857

Host		E Coli

Purity/Purification		> 95%
Form/Format		Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.

Organism		Mus musculus (Mouse)
Expression System		Prokaryotic expression
Residues		His560~Pro911 (Accession # P97857) with N-terminal His-Tag
Endotoxin Level		<1.0EU per 1ug (determined by the LAL method)
Reconstitution		Reconstitute in sterile PBS, pH7.2-pH7.4.

Preparation and Storage		Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months. Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.
Other Notes		Small volumes of ADAMTS1 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Product Description specifically for ADAMTS1 recombinant protein	About the Marker: Effective Size Range: 10kDa to 70kDa. Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and70kDa. Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at doubleintensity to make location and size approximation of proteins of interestquick and easy. Ready-to-use: No need to heat, dilute or add reducing agents before use.

Applications Tested/Suitable forADAMTS1 recombinant protein	SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
TOP

*NCBI/Uniprot data below describe general gene information for ADAMTS1. It may not necessarily be applicable to this product.*
NCBI GI #	157951646
NCBI GeneID	11504
NCBI Accession #	NP_033751.3	[Other Products]
NCBI GenBank Nucleotide #	NM_009621.4	[Other Products]

UniProt Primary Accession #	P97857	[Other Products]
UniProt Secondary Accession #	O54768; E9QMN9	[Other Products]
UniProt Related Accession #	P97857	[Other Products]
Molecular Weight	40.2kDa