Recombinant A Disintegrin And Metalloprotease 12 (ADAM12)

Instruction Manual!

Product name:	Recombinant A Disintegrin And Metalloprotease 12 (ADAM12)
Source:	Prokaryotic expression
Purity:	>95%
Buffer Formulation:	Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
Applications:	SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
Storage:	Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.
UOM:	10ug/50ug/100ug/200ug/1mg

Product Name   A Disintegrin And Metalloprotease 12 (ADAM12), Recombinant Protein
Also Known As   Recombinant A Disintegrin And Metalloprotease 12 (ADAM12)
Product Gene Name   ADAM12 recombinant protein [Similar Products]
Research Use Only   For Research Use Only. Not for use in diagnostic procedures

Sequence    The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.
SCQDVDGY CYNGICQTHE QQCVTLWGPG AKPAPGICFE RVNSAGDPYG NCGKVSKSSF AKCEMRDAKC GKIQCQGGAS RPVIGTNAVS IETNIPLQQG GRILCRGTHV YLGDDMPDPG LVLAGTKCAD GKICLNRQCQ NISVFGVHEC AMQCHGRGVC NNRKNCHCEA HWAPPFCDKF GFGGSTDSGP IRQADNQGLT IGILVTILCL LAAGFVVYLK RKTLIRLLFT NKKTTIEKLR CVRPSRPPRG FQPCQAH

Host    Host: E Coli
Source: Prokaryotic expression

Purity/Purification    >95%

Form/Format    Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.

Predicted Molecular Mass    31.3kDa

Accurate Molecular Mass (KD)    31kDa

Endotoxin    <1.0EU per 1ug (determined by the LAL method)

Expression System    Prokaryotic expression

Tag    two N-terminal tags, his-tag and T7 tag

Organism Species    Homo sapiens (Human)

Fragment    Ser513~His767

Usage    Reconstitute in sterile PBS, pH7.2-pH7.4.

Preparation and Storage    Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.
Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. The loss of this protein is less than 5% within the expiration date under appropriate storage condition.

Other Notes    Small volumes of ADAM12 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Applications Tested/Suitable forADAM12 recombinant protein
SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)

Sequence		The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below. SCQDVDGY CYNGICQTHE QQCVTLWGPG AKPAPGICFE RVNSAGDPYG NCGKVSKSSF AKCEMRDAKC GKIQCQGGAS RPVIGTNAVS IETNIPLQQG GRILCRGTHV YLGDDMPDPG LVLAGTKCAD GKICLNRQCQ NISVFGVHEC AMQCHGRGVC NNRKNCHCEA HWAPPFCDKF GFGGSTDSGP IRQADNQGLT IGILVTILCL LAAGFVVYLK RKTLIRLLFT NKKTTIEKLR CVRPSRPPRG FQPCQAH

Host		Host: E Coli Source: Prokaryotic expression

Purity/Purification		>95%
Form/Format		Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.

Predicted Molecular Mass		31.3kDa
Accurate Molecular Mass (KD)		31kDa
Endotoxin		<1.0EU per 1ug (determined by the LAL method)
Expression System		Prokaryotic expression
Tag		two N-terminal tags, his-tag and T7 tag
Organism Species		Homo sapiens (Human)
Fragment		Ser513~His767
Usage		Reconstitute in sterile PBS, pH7.2-pH7.4.

Preparation and Storage		Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months. Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
Other Notes		Small volumes of ADAM12 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.