Recombinant A Disintegrin And Metalloprotease 17 (ADAM17)

Instruction Manual!

Product name:	Recombinant A Disintegrin And Metalloprotease 17 (ADAM17)
Source:	Prokaryotic expression
Purity:	> 95%
Buffer Formulation:	Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
Applications:	SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
Storage:	Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.
UOM:	10ug/50ug/100ug/200ug/1mg

Product Name   A Disintegrin And Metalloprotease 17 (ADAM17), Recombinant Protein
Also Known As   Recombinant A Disintegrin And Metalloprotease 17 (ADAM17)
Product Gene Name   ADAM17 recombinant protein [Similar Products]
Research Use Only   For Research Use Only. Not for use in diagnostic procedures.

Sequence    The target protein is fused with N-terminal His-Tag, its sequence is listed below.
MGHHHHHHSG SEF-KLLVVA D HRFYRYMGRG EESTTTNYLI ELIDRVDDIY RNTSWDNAGF KGYGIQIEQI RILKSPQEVK PGEKHYNMAK SYPNEEKDAW DVKMLLEQFS FDIAEEASKV CLAHLFTYQD FDMGTLGLAY VGSPRANSHG GVCPKAYYSP VGKKNIYLNS GLTSTKNYGK TILTKEADLV TTHELGHNFG AEHDPDGLAE CAPNEDQGGK YVMYPIAVSG DHENNKMFSN CSKQSIYKTI ESKAQECFQE R

Chromosome Location    Chromosome: 2; NC_000002.11 (9629392..9695917, complement). Location: 2p25

OMIM    603639

3D Structure    ModBase 3D Structure for P78536

Host    E Coli

Purity/Purification    > 95%

Form/Format    Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.

Organism    Homo sapiens (Human)

Expression System    Prokaryotic expression

Residues    Lys226~Arg473 (Accession # P78536) with N-terminal His-Tag

Endotoxin Level    <1.0EU per 1ug (determined by the LAL method)

Reconstitution    Reconstitute in sterile PBS, pH7.2-pH7.4.

Preparation and Storage    Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.
Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

Other Notes    Small volumes of ADAM17 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Product Description specifically for ADAM17 recombinant protein
   About the Marker: Effective Size Range: 10kDa to 70kDa.
Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and70kDa.
Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at doubleintensity to make location and size approximation of proteins of interestquick and easy.
Ready-to-use: No need to heat, dilute or add reducing agents before use.

Applications Tested/Suitable forADAM17 recombinant protein
SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)

SDS-PAGE of ADAM17 recombinant protein

Sequence		The target protein is fused with N-terminal His-Tag, its sequence is listed below. MGHHHHHHSG SEF-KLLVVA D HRFYRYMGRG EESTTTNYLI ELIDRVDDIY RNTSWDNAGF KGYGIQIEQI RILKSPQEVK PGEKHYNMAK SYPNEEKDAW DVKMLLEQFS FDIAEEASKV CLAHLFTYQD FDMGTLGLAY VGSPRANSHG GVCPKAYYSP VGKKNIYLNS GLTSTKNYGK TILTKEADLV TTHELGHNFG AEHDPDGLAE CAPNEDQGGK YVMYPIAVSG DHENNKMFSN CSKQSIYKTI ESKAQECFQE R
Chromosome Location		Chromosome: 2; NC_000002.11 (9629392..9695917, complement). Location: 2p25
OMIM		603639
3D Structure		ModBase 3D Structure for P78536

Host		E Coli

Purity/Purification		> 95%
Form/Format		Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.

Organism		Homo sapiens (Human)
Expression System		Prokaryotic expression
Residues		Lys226~Arg473 (Accession # P78536) with N-terminal His-Tag
Endotoxin Level		<1.0EU per 1ug (determined by the LAL method)
Reconstitution		Reconstitute in sterile PBS, pH7.2-pH7.4.

Preparation and Storage		Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months. Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.
Other Notes		Small volumes of ADAM17 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Product Description specifically for ADAM17 recombinant protein		About the Marker: Effective Size Range: 10kDa to 70kDa. Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and70kDa. Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at doubleintensity to make location and size approximation of proteins of interestquick and easy. Ready-to-use: No need to heat, dilute or add reducing agents before use.

Applications Tested/Suitable forADAM17 recombinant protein		SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)

SDS-PAGE of ADAM17 recombinant protein