Recombinant A Disintegrin And Metalloprotease 10 (ADAM10)

Instruction Manual!

Product name:	Recombinant A Disintegrin And Metalloprotease 10 (ADAM10)
Source:	Prokaryotic expression
Purity:	> 95%
Buffer Formulation:	Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
Applications:	SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
Storage:	Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.
UOM:	10ug/50ug/100ug/200ug/1mg

Product Name A Disintegrin And Metalloprotease 10 (ADAM10), Recombinant Protein
Also Known As Recombinant A Disintegrin And Metalloprotease 10 (ADAM10)
Product Gene Name ADAM10 recombinant protein [Similar Products]
Research Use Only For Research Use Only. Not for use in diagnostic procedures.

Sequence    The target protein is fused with N-terminal His-Tag, its sequence is listed below.
MGHHHHHHSG SEF-TSG DKLNNNKFSL CSIRNISQVL EKKRNNCFVE SGQPICGNGM VEQGEECDCG YSDQCKDECC FDANQPEGKK CKLKPGKQCS PSQGPCCTAQ CAFKSKSEKC RDDSDCAKEG ICNGFTALCP ASDPKPNFTD CNRHTQVCIN GQCAGSICEK YDLEECTCAS SDGKDDKELC HVCCMKKMAP STCASTGSLQ WNKQFTGRTI TLQPGSPCND FRGYCDVFMR CRLVD

Chromosome Location    Chromosome: 8; NC_005107.3 (75842118..75974564, complement). Location: 8q24

3D Structure    ModBase 3D Structure for Q10743

Host    E Coli

Purity/Purification    > 95%

Form/Format    Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.

Organism    Rattus norvegicus (Rat)

Expression System    Prokaryotic expression

Residues    Thr218~Asp445 (Accession # Q10743) with N-terminal His-Tag

Subcellular Location    Membrane; Single-pass type I membrane protein

Endotoxin Level    <1.0EU per 1ug (determined by the LAL method)

Predicted Isoelectric Point    6.4

Reconstitution    Reconstitute in sterile PBS, pH7.2-pH7.4.

Preparation and Storage    Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.
Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

Other Notes    Small volumes of ADAM10 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Product Description specifically for ADAM10 recombinant protein
   About the Marker: Effective Size Range: 10kDa to 70kDa.
Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and70kDa.
Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at doubleintensity to make location and size approximation of proteins of interestquick and easy.
Ready-to-use: No need to heat, dilute or add reducing agents before use.

Applications Tested/Suitable forADAM10 recombinant protein
SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)

SDS-PAGE of ADAM10 recombinant protein

Sequence		The target protein is fused with N-terminal His-Tag, its sequence is listed below. MGHHHHHHSG SEF-TSG DKLNNNKFSL CSIRNISQVL EKKRNNCFVE SGQPICGNGM VEQGEECDCG YSDQCKDECC FDANQPEGKK CKLKPGKQCS PSQGPCCTAQ CAFKSKSEKC RDDSDCAKEG ICNGFTALCP ASDPKPNFTD CNRHTQVCIN GQCAGSICEK YDLEECTCAS SDGKDDKELC HVCCMKKMAP STCASTGSLQ WNKQFTGRTI TLQPGSPCND FRGYCDVFMR CRLVD
Chromosome Location		Chromosome: 8; NC_005107.3 (75842118..75974564, complement). Location: 8q24
3D Structure		ModBase 3D Structure for Q10743

Host		E Coli

Purity/Purification		> 95%
Form/Format		Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.

Organism		Rattus norvegicus (Rat)
Expression System		Prokaryotic expression
Residues		Thr218~Asp445 (Accession # Q10743) with N-terminal His-Tag
Subcellular Location		Membrane; Single-pass type I membrane protein
Endotoxin Level		<1.0EU per 1ug (determined by the LAL method)
Predicted Isoelectric Point		6.4
Reconstitution		Reconstitute in sterile PBS, pH7.2-pH7.4.

Preparation and Storage		Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months. Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.
Other Notes		Small volumes of ADAM10 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Product Description specifically for ADAM10 recombinant protein		About the Marker: Effective Size Range: 10kDa to 70kDa. Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and70kDa. Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at doubleintensity to make location and size approximation of proteins of interestquick and easy. Ready-to-use: No need to heat, dilute or add reducing agents before use.

Applications Tested/Suitable forADAM10 recombinant protein		SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)

SDS-PAGE of ADAM10 recombinant protein