Recombinant A Disintegrin And Metalloprotease 22 (ADAM22)

Instruction Manual!

Product name:	Recombinant A Disintegrin And Metalloprotease 22 (ADAM22)
Source:	Prokaryotic expression
Purity:	> 95%
Buffer Formulation:	Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
Applications:	SDS-PAGE, Western (WB), ELISA (EIA), Immunoprecipitation (IP).
Storage:	Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.
UOM:	10ug/50ug/100ug/200ug/1mg

Product Name A Disintegrin And Metalloprotease 22 (ADAM22), Recombinant Protein
Also Known As Recombinant A Disintegrin And Metalloprotease 22 (ADAM22)
Product Synonym Names MDC2; Disintegrin And Metalloproteinase Domain-Containing Protein 22; Metalloproteinase-Like,Disintegrin-Like,And Cysteine-Rich Protein 2
Product Gene Name ADAM22 recombinant protein [Similar Products]
Research Use Only For Research Use Only. Not for use in diagnostic procedures.

Sequence    L AQSLAHNIGI ISDKRKLASG ECKCEDTWSG CIMGDTGYYL PKKFTQCNIE EYHDFLNSGG GACLFNKPSK LLDPPECGNG FIETGEECDC GTPAECVLEG AECCKKCTLT QDSQCSDGLC CKKCKFQPMG TVCREAVNDC DIRETCSGNS SQCAPNIHKM DGYSCDGVQG ICFGGRCKTR DRQCKYIWGQ KVTASDKYCY EKLNIEGTEK GN

OMIM    603709

Host    E Coli

Purity/Purification    > 95%

Form/Format    Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.

Predicted Molecular Mass    27.0kDa

Usage    Reconstitute in sterile PBS, pH7.2-pH7.4.

Endotoxin Level    <1.0EU per 1 ug (determined by the LAL method)

Expression System    Prokaryotic expression

Fragment    Leu370~Asn582

Organism Species    Human

Tag    two N-terminal Tags, His-tag and T7-tag

Preparation and Storage    Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.
Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

Other Notes    Small volumes of ADAM22 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Applications Tested/Suitable forADAM22 recombinant protein
SDS-PAGE, Western (WB), ELISA (EIA), Immunoprecipitation (IP).

SDS-Page of ADAM22 recombinant protein

Sequence		L AQSLAHNIGI ISDKRKLASG ECKCEDTWSG CIMGDTGYYL PKKFTQCNIE EYHDFLNSGG GACLFNKPSK LLDPPECGNG FIETGEECDC GTPAECVLEG AECCKKCTLT QDSQCSDGLC CKKCKFQPMG TVCREAVNDC DIRETCSGNS SQCAPNIHKM DGYSCDGVQG ICFGGRCKTR DRQCKYIWGQ KVTASDKYCY EKLNIEGTEK GN
OMIM		603709

Host		E Coli

Purity/Purification		> 95%
Form/Format		Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.

Predicted Molecular Mass		27.0kDa
Usage		Reconstitute in sterile PBS, pH7.2-pH7.4.
Endotoxin Level		<1.0EU per 1 ug (determined by the LAL method)
Expression System		Prokaryotic expression
Fragment		Leu370~Asn582
Organism Species		Human
Tag		two N-terminal Tags, His-tag and T7-tag

Preparation and Storage		Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months. Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.
Other Notes		Small volumes of ADAM22 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Applications Tested/Suitable forADAM22 recombinant protein		SDS-PAGE, Western (WB), ELISA (EIA), Immunoprecipitation (IP).

SDS-Page of ADAM22 recombinant protein