Recombinant A Disintegrin And Metalloprotease 28 (ADAM28)

Instruction Manual!

Product name:	Recombinant A Disintegrin And Metalloprotease 28 (ADAM28)
Source:	Prokaryotic expression
Purity:	>98%
Buffer Formulation:	20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5%Trehalose and Proclin300
Applications:	SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP), CoIP, ReporterAssays, Purification, Amine Reactive Labeling.
Storage:	Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.
UOM:	10ug/50ug/100ug/200ug/1mg

Product Name A Disintegrin And Metalloprotease 28 (ADAM28), Recombinant Protein
Also Known As Recombinant A Disintegrin And Metalloprotease 28 (ADAM28)
Product Gene Name ADAM28 recombinant protein [Similar Products]
Research Use Only For Research Use Only. Not for use in diagnostic procedures.

Sequence    The target protein is fused with N-terminal His-Tag, its sequence is listed below.
MGHHHHHHSG SEF-EVADKS CYNRNEGGSK YGYCRRVDDT LIPCKANDTM CGKLFCQGGS DNLPWKGRIV TFLTCKTFDP EDTSQEIGMV ANGTKCGDNK VCINAECVDI EKAYKSTNCS SKCKGHAVCD HELQCQCEEG WIPPDCDDSS VVFHFSIVVG VLFPMAVIFV VVAMVIRHQS SREKQKKDQR PLSTTGTRPH KQKRKPQMVK AVQPQEMSQM KPHVYDLPVE GNEPPASFHK DTNALPPTVF KDNPVSTPKD SNPK

Chromosome Location    Chromosome: 8; NC_000008.10 (24151553..24212726). Location: 8p21.2

OMIM    606188

3D Structure    ModBase 3D Structure for Q9UKQ2

Host    E Coli

Purity/Purification    >98%

Form/Format    20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5%Trehalose and Proclin300

Organism    Homo sapiens (Human)

Expression System    Prokaryotic expression

Residues    Glu525-Lys774 (Accession # Q9UKQ2) with N-terminal His-Tag

Tissue Specificity    Spleen, Stomach, Intestine, Brain.

Subcellular Location    Cell membrane; Single-pass type I membrane protein.

Traits    Freeze-dried powder

Predicted isoelectric point    7.5

Predicted Molecular Mass    29.3kDa

Accurate Molecular Mass    34kDa as determined by SDS-PAGE reducing conditions.

Phenomenon explanation    The possible reasons that the actual band size differs from the predicted are as follows:
1. Splice variants: Alternative splicing may create different sized proteins from the same gene.
2. Relative charge: The composition of amino acids may affects the charge of the protein.
3. Post-translational modification: Phosphorylation, glycosylation, methylation etc.
4. Post-translation cleavage: Many proteins are synthesized as pro-proteins, and then cleaved to give the active form.
5. Polymerization of the target protein: Dimerization, multimerization etc.

Endotoxin Level    <1.0EU per 1ug (determined by the LAL method)

Reconstitution    Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Preparation and Storage    Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.
Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

Other Notes    Small volumes of ADAM28 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Applications Tested/Suitable for ADAM28 recombinant protein
SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP), CoIP, ReporterAssays, Purification, Amine Reactive Labeling.

SDS-PAGE ofADAM28 recombinant protein

Sequence		The target protein is fused with N-terminal His-Tag, its sequence is listed below. MGHHHHHHSG SEF-EVADKS CYNRNEGGSK YGYCRRVDDT LIPCKANDTM CGKLFCQGGS DNLPWKGRIV TFLTCKTFDP EDTSQEIGMV ANGTKCGDNK VCINAECVDI EKAYKSTNCS SKCKGHAVCD HELQCQCEEG WIPPDCDDSS VVFHFSIVVG VLFPMAVIFV VVAMVIRHQS SREKQKKDQR PLSTTGTRPH KQKRKPQMVK AVQPQEMSQM KPHVYDLPVE GNEPPASFHK DTNALPPTVF KDNPVSTPKD SNPK
Chromosome Location		Chromosome: 8; NC_000008.10 (24151553..24212726). Location: 8p21.2
OMIM		606188
3D Structure		ModBase 3D Structure for Q9UKQ2

Host		E Coli

Purity/Purification		>98%
Form/Format		20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5%Trehalose and Proclin300

Organism		Homo sapiens (Human)
Expression System		Prokaryotic expression
Residues		Glu525-Lys774 (Accession # Q9UKQ2) with N-terminal His-Tag
Tissue Specificity		Spleen, Stomach, Intestine, Brain.
Subcellular Location		Cell membrane; Single-pass type I membrane protein.
Traits		Freeze-dried powder
Predicted isoelectric point		7.5
Predicted Molecular Mass		29.3kDa
Accurate Molecular Mass		34kDa as determined by SDS-PAGE reducing conditions.
Phenomenon explanation		The possible reasons that the actual band size differs from the predicted are as follows: 1. Splice variants: Alternative splicing may create different sized proteins from the same gene. 2. Relative charge: The composition of amino acids may affects the charge of the protein. 3. Post-translational modification: Phosphorylation, glycosylation, methylation etc. 4. Post-translation cleavage: Many proteins are synthesized as pro-proteins, and then cleaved to give the active form. 5. Polymerization of the target protein: Dimerization, multimerization etc.
Endotoxin Level		<1.0EU per 1ug (determined by the LAL method)
Reconstitution		Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Preparation and Storage		Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months. Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.
Other Notes		Small volumes of ADAM28 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Applications Tested/Suitable for ADAM28 recombinant protein		SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP), CoIP, ReporterAssays, Purification, Amine Reactive Labeling.

SDS-PAGE ofADAM28 recombinant protein