Recombinant A Disintegrin And Metalloprotease 8 (ADAM8)

Instruction Manual!

Product name:	Recombinant A Disintegrin And Metalloprotease 8 (ADAM8)
Source:	Prokaryotic expression
Purity:	>95%
Buffer Formulation:	Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
Applications:	SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
Storage:	Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.
UOM:	10ug/50ug/100ug/200ug/1mg

Product Name A Disintegrin And Metalloprotease 8 (ADAM8), Recombinant Protein
Also Known As Recombinant A Disintegrin And Metalloprotease 8 (ADAM8)
Product Gene Name ADAM8 recombinant protein [Similar Products]
Research Use Only For Research Use Only. Not for use in diagnostic procedures.

Sequence    The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.
MGSSHHHHH H SSGLVPRGSH MASMTGGQQM GRGSEF- EEGQHA MYQAKHLQQK AGTCGVKDTN LNDLGPRALE IYRAQPRNWL IPRETRYVEL YVVADSQEFQ KLGSREAVRQ RVLEVVNHVD KLYQELSFRV VLVGLEIWNK DKFYISRYAN VTLENFLSWR EQNLQGQHPH DNVQLITGVD FIGSTVGLAK VSALCSRHSG AVNQDHSKNS IGVASTMAHE LGHNLGMSHD EDIPGCYCPE PREGGGCIMT ESIGSKFPRI FSRCSKIDLE SFVTKPQTGC LTNVPDVNRF VGGPVCGNLF VEHGEQCDCG TPQDCQNPCC NATTCQLVKG AECASGTCCH ECKVKPAGEV CRLSKDKCDL EEFCDGRKPT CPEDAFQQNG TPC

Host    Host: E Coli
Source: Prokaryotic expression

Purity/Purification    >95%

Form/Format    Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.

Concentration    6.3 (lot specific)

Predicted Molecular Mass    42.5kDa

Accurate Molecular Mass (KD)    44kDa

Endotoxin    <1.0EU per 1ug (determined by the LAL method)

Expression System    Prokaryotic expression

Tag    two N-terminal Tags, His-tag and T7-tag

Organism Species    Mus musculus (Mouse)

Fragment    Glu145~Cys493

Usage    Reconstitute in sterile PBS, pH7.2-pH7.4.

Preparation and Storage    Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.
Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. The loss of this protein is less than 5% within the expiration date under appropriate storage condition.

Other Notes    Small volumes of ADAM8 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Applications Tested/Suitable for ADAM8 recombinant protein
SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)

SDS-Page of ADAM8 recombinant protein

Sequence		The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below. MGSSHHHHH H SSGLVPRGSH MASMTGGQQM GRGSEF- EEGQHA MYQAKHLQQK AGTCGVKDTN LNDLGPRALE IYRAQPRNWL IPRETRYVEL YVVADSQEFQ KLGSREAVRQ RVLEVVNHVD KLYQELSFRV VLVGLEIWNK DKFYISRYAN VTLENFLSWR EQNLQGQHPH DNVQLITGVD FIGSTVGLAK VSALCSRHSG AVNQDHSKNS IGVASTMAHE LGHNLGMSHD EDIPGCYCPE PREGGGCIMT ESIGSKFPRI FSRCSKIDLE SFVTKPQTGC LTNVPDVNRF VGGPVCGNLF VEHGEQCDCG TPQDCQNPCC NATTCQLVKG AECASGTCCH ECKVKPAGEV CRLSKDKCDL EEFCDGRKPT CPEDAFQQNG TPC

Host		Host: E Coli Source: Prokaryotic expression

Purity/Purification		>95%
Form/Format		Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
Concentration		6.3 (lot specific)

Predicted Molecular Mass		42.5kDa
Accurate Molecular Mass (KD)		44kDa
Endotoxin		<1.0EU per 1ug (determined by the LAL method)
Expression System		Prokaryotic expression
Tag		two N-terminal Tags, His-tag and T7-tag
Organism Species		Mus musculus (Mouse)
Fragment		Glu145~Cys493
Usage		Reconstitute in sterile PBS, pH7.2-pH7.4.

Preparation and Storage		Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months. Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
Other Notes		Small volumes of ADAM8 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Applications Tested/Suitable for ADAM8 recombinant protein		SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)

SDS-Page of ADAM8 recombinant protein