Recombinant A Disintegrin And Metalloproteinase With Thrombospondin 7 (ADAMTS7)

Instruction Manual!

Product name:	Recombinant A Disintegrin And Metalloproteinase With Thrombospondin 7 (ADAMTS7)
Source:	Prokaryotic expression
Purity:	> 95%
Buffer Formulation:	Supplied as lyophilized form in PBS, pH7.4, containing 1mM DTT, 5% trehalose, 0.01% sarcosyl and preservative.
Applications:	SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
Storage:	Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.
UOM:	10ug/50ug/100ug/200ug/1mg

Product Name A Disintegrin And Metalloproteinase With Thrombospondin 7 (ADAMTS7), Recombinant Protein
Also Known As Recombinant A Disintegrin And Metalloproteinase With Thrombospondin 7 (ADAMTS7)
Product Gene Name ADAMTS7 recombinant protein [Similar Products]
Research Use Only For Research Use Only. Not for use in diagnostic procedures.

Sequence    The target protein is fused with N-terminal His-Tag, its sequence is listed below.
MGHHHHHHSG SEF-VQ RRLVKCVNTQ TGLPEEDSDQ CGHEAWPESS RPCGTEDCEP VEPPRCERDR LSFGFCETLR LLGRCQLPTI RTQCCRSCSP PSHGAPSRGH QRVARR

Chromosome Location    Chromosome: 15; NC_000015.9 (79051545..79103805, complement). Location: 15q24.2

OMIM    605009

3D Structure    ModBase 3D Structure for Q9UKP4

Host    E Coli

Purity/Purification    > 95%

Form/Format    Supplied as lyophilized form in PBS, pH7.4, containing 1mM DTT, 5% trehalose, 0.01% sarcosyl and preservative.

Residues    Val1589~Arg1686

Tags Information    N-terminal His-Tag

Endotoxin Level    <1.0EU per 1ug(determined by the LAL method).

Predicted isoelectric point    7.6

Reconstitution    Reconstitute in sterile PBS, pH7.2-pH7.4.

Preparation and Storage    Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.
Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

Other Notes    Small volumes of ADAMTS7 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Applications Tested/Suitable for ADAMTS7 recombinant protein
SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)

SDS-PAGE of ADAMTS7 recombinant protein

Sequence		The target protein is fused with N-terminal His-Tag, its sequence is listed below. MGHHHHHHSG SEF-VQ RRLVKCVNTQ TGLPEEDSDQ CGHEAWPESS RPCGTEDCEP VEPPRCERDR LSFGFCETLR LLGRCQLPTI RTQCCRSCSP PSHGAPSRGH QRVARR
Chromosome Location		Chromosome: 15; NC_000015.9 (79051545..79103805, complement). Location: 15q24.2
OMIM		605009
3D Structure		ModBase 3D Structure for Q9UKP4

Host		E Coli

Purity/Purification		> 95%
Form/Format		Supplied as lyophilized form in PBS, pH7.4, containing 1mM DTT, 5% trehalose, 0.01% sarcosyl and preservative.

Residues		Val1589~Arg1686
Tags Information		N-terminal His-Tag
Endotoxin Level		<1.0EU per 1ug(determined by the LAL method).
Predicted isoelectric point		7.6
Reconstitution		Reconstitute in sterile PBS, pH7.2-pH7.4.

Preparation and Storage		Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months. Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.
Other Notes		Small volumes of ADAMTS7 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Applications Tested/Suitable for ADAMTS7 recombinant protein		SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)

SDS-PAGE of ADAMTS7 recombinant protein