Recombinant Human Protein AATF

Instruction Manual!

Product name:	Recombinant Human Protein AATF
Source:
Purity:
Buffer Formulation:	Greater than 90% as determined by SDS-PAGE.
Applications:	May function as a general inhibitor of the histone deacetylase HDAC1. Binding to the pocket region of RB1 may displace HDAC1 from RB1/E2F complexes, leading to activation of E2F target genes and cell cycle progression. Conversely, displacent of HDAC1 from
Storage:	Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.
UOM:	10ug/50ug/100ug/200ug/1mg

Product Name AATF, Recombinant Protein
Also Known As Recombinant Human Protein AATF
Product Synonym Names Apoptosis-antagonizing transcription factor; Rb-binding protein Che-1
Product Gene Name AATF recombinant protein [Similar Products]
Research Use Only For Research Use Only. Not for use in diagnostic procedures.
Matching Pairs Antibody: AATF (MBS7041862)
Standard: AATF (MBS717108)
Comments: Standards are recommendations only. Compatibility of the standards in the user developed application needs to be established and confirmed by the researcher.

Sequence Positions    6-294

Sequence    PLALQLEQLL NPRPSEADPE ADPEEATAAR VIDRFDEGED GEGDFLVVGS IRKLASASLL DTDKRYCGKT TSRKAWNEDH WEQTLPGSSD EEISDEEGSG DEDSEGLGLE EYDEDDLGAA EEQECGDHRE SKKSRSHSAK TPGFSVQSIS DFEKFTKGMD DLGSSEEEED EESGMEEGDD AEDSQGESEE DRAGDRNSED DGVVMTFSSV KVSEEVEKGR AVKNQIALWD QLLEGRIKLQ KALLTTNQLP QPDVF

OMIM    608463

3D Structure    ModBase 3D Structure for Q9NY61

Host    E Coli

Purity/Purification    Greater than 90% as determined by SDS-PAGE.

Tag Information    This protein contains an N-terminal tag and may also contain a C-terminal Myc-tag. N-terminal host tags may vary (His, His-SUMO, His-B2M, GST). Tag types are determined by various factors including tag-protein stability and, therefore, are subject to change; please inquire for tag information. Additional charge for Tag removal. Tag removal service standardly refers to removal of N-terminal Tag. Any C-terminal Myc-tag may remain intact unless removal of the C-terminal Myc-tag is specifically requested. In most cases tags can be successfully removed. If the lab concludes the protein is no longer stable after tag removal then your protein will be supplied with the tag intact, and you will not be charged for tag removal service.

Sterility    Sterile filter available upon request.

Endotoxin    Low endotoxin available upon request.

Preparation and Storage    Store at -20 degree C, for extended storage, conserve at -20 degree C or -80 degree C.

ISO Certification    Manufactured in an ISO 9001:2008 Certified Laboratory.

Other Notes    Small volumes of AATF recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Product Description specifically for AATF recombinant protein
   May function as a general inhibitor of the histone deacetylase HDAC1. Binding to the pocket region of RB1 may displace HDAC1 from RB1/E2F complexes, leading to activation of E2F target genes and cell cycle progression. Conversely, displacent of HDAC1 from SP1 bound to the CDKN1A promoter leads to increased expression of this CDK inhibitor and blocks cell cycle progression. Also antagonizes PAWR mediated induction of aberrant amyloid peptide production in Alzheimer disease (presenile and senile dentia), although the molecular basis for this phenomenon has not been described to date.

Product Categories/Family forAATF recombinant protein    Epigenetics and Nuclear Signaling

SDS-Page of AATF recombinant protein

Sequence Positions		6-294
Sequence		PLALQLEQLL NPRPSEADPE ADPEEATAAR VIDRFDEGED GEGDFLVVGS IRKLASASLL DTDKRYCGKT TSRKAWNEDH WEQTLPGSSD EEISDEEGSG DEDSEGLGLE EYDEDDLGAA EEQECGDHRE SKKSRSHSAK TPGFSVQSIS DFEKFTKGMD DLGSSEEEED EESGMEEGDD AEDSQGESEE DRAGDRNSED DGVVMTFSSV KVSEEVEKGR AVKNQIALWD QLLEGRIKLQ KALLTTNQLP QPDVF
OMIM		608463
3D Structure		ModBase 3D Structure for Q9NY61

Host		E Coli

Purity/Purification		Greater than 90% as determined by SDS-PAGE.
Tag Information		This protein contains an N-terminal tag and may also contain a C-terminal Myc-tag. N-terminal host tags may vary (His, His-SUMO, His-B2M, GST). Tag types are determined by various factors including tag-protein stability and, therefore, are subject to change; please inquire for tag information. Additional charge for Tag removal. Tag removal service standardly refers to removal of N-terminal Tag. Any C-terminal Myc-tag may remain intact unless removal of the C-terminal Myc-tag is specifically requested. In most cases tags can be successfully removed. If the lab concludes the protein is no longer stable after tag removal then your protein will be supplied with the tag intact, and you will not be charged for tag removal service.
Sterility		Sterile filter available upon request.
Endotoxin		Low endotoxin available upon request.


Preparation and Storage		Store at -20 degree C, for extended storage, conserve at -20 degree C or -80 degree C.
ISO Certification		Manufactured in an ISO 9001:2008 Certified Laboratory.
Other Notes		Small volumes of AATF recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Product Description specifically for AATF recombinant protein		May function as a general inhibitor of the histone deacetylase HDAC1. Binding to the pocket region of RB1 may displace HDAC1 from RB1/E2F complexes, leading to activation of E2F target genes and cell cycle progression. Conversely, displacent of HDAC1 from SP1 bound to the CDKN1A promoter leads to increased expression of this CDK inhibitor and blocks cell cycle progression. Also antagonizes PAWR mediated induction of aberrant amyloid peptide production in Alzheimer disease (presenile and senile dentia), although the molecular basis for this phenomenon has not been described to date.
Product Categories/Family forAATF recombinant protein		Epigenetics and Nuclear Signaling


SDS-Page of AATF recombinant protein