Recombinant Peptidylglycine Alpha Amidating Monooxygenase (PAM)

Instruction Manual!

Product name:	Recombinant Peptidylglycine Alpha Amidating Monooxygenase (PAM)
Source:	Homo sapiens (Human)
Purity:	>90%
Buffer Formulation:	Reconstitute in sterile PBS, pH7.2-pH7.4.
Applications:	SDS-PAGE; WB; ELISA; IP.
Storage:	Avoid repeated freeze/thaw cycles.
UOM:	10ug/50ug/100ug/200ug/1mg

[ PROPERTIES ]

Residues: Phe21~Cys288

Tags: Two N-terminal Tags, His-tag and T7-tag

Accession: P19021

Host: E. coli

Subcellular Location: Membrane. Secreted.

Purity: >90%

Endotoxin Level: <1.0EU per 1μg (determined by the LAL method).

Formulation: Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.

Predicted isoelectric point: 8.8

Predicted Molecular Mass: 33.7kDa

Applications: SDS-PAGE; WB; ELISA; IP.

(May be suitable for use in other assays to be determined by the end user.)

[ USAGE ]

Reconstitute in sterile PBS, pH7.2-pH7.4.

[ STORAGE AND STABILITY ]

Storage: Avoid repeated freeze/thaw cycles.

Store at 2-8oC for one month.

Aliquot and store at -80oC for 12 months.

Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.

[ SEQUENCES ]

The sequence of the target protein is listed below.

FRSPLSVFKR FKETTRPFSN ECLGTTRPVV PIDSSDFALD IRMPGVTPKQ SDTYFCMSMR

IPVDEEAFVI DFKPRASMDT VHHMLLFGCN MPSSTGSYWF CDEGTCTDKA NILYAWARNA

PPTRLPKGVG FRVGGETGSK YFVLQVHYGD ISAFRDNNKD CSGVSLHLTR LPQPLIAGMY

LMMSVDTVIP AGEKVVNSDI SCHYKNYPMH VFAYRVHTHH LGKVVSGYRV RNGQWTLIGR