ACADM, 26-421aa, Human, His tag, E Coli

Instruction Manual!

Product name:	ACADM, 26-421aa, Human, His tag, E Coli
Source:	E Coli
Purity:	>90%
Buffer Formulation:	Liquid. 20mM Tris-HCl buffer (pH8.0) containing 20% glycerol 0.1M NaCl
Applications:	SDS-PAGE
Storage:	Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.
UOM:	10ug/50ug/100ug/200ug/1mg

Product Name ACADM, Recombinant Protein
Also Known As ACADM, 26-421aa, Human, His tag, E Coli
Product Synonym Names ACAD1; CAD; MCADH; Medium-chain specific acyl-CoA dehydrogenase; ACAD 1; Acyl Coenzyme A Dehydrogenase; Acyl coenzyme A dehydrogenase C 4 to C 12 straight chain; C4 to C12 Straight Chain; FLJ18227; FLJ93013; FLJ99884; MCAD; Medium chain acyl CoA dehydrogenase; Medium chain fatty acyl CoA dehydrogenase; Medium chain specific acyl CoA dehydrogenase; Medium chain specific acyl CoA dehydrogenase mitochondrial
Product Gene Name ACADM recombinant protein [Similar Products]
Research Use Only For Research Use Only. Not for use in diagnostic procedures.

Sequence    MGSSHHHHHH SSGLVPRGSH MKANRQREPG LGFSFEFTEQ QKEFQATARK FAREEIIPVA AEYDKTGEYP VPLIRRAWEL GLMNTHIPEN CGGLGLGTFD ACLISEELAY GCTGVQTAIE GNSLGQMPII IAGNDQQKKK YLGRMTEEPL MCAYCVTEPG AGSDVAGIKT KAEKKGDEYI INGQKMWITN GGKANWYFLL ARSDPDPKAP ANKAFTGFIV EADTPGIQIG RKELNMGQRC SDTRGIVFED VKVPKENVLI GDGAGFKVAM GAFDKTRPVV AAGAVGLAQR ALDEATKYAL ERKTFGKLLV EHQAISFMLA EMAMKVELAR MSYQRAAWEV DSGRRNTYYA SIAKAFAGDI ANQLATDAVQ ILGGNGFNTE YPVEKLMRDA KIYQIYEGTS QIQRLIVARE HIDKYKN

OMIM    Online Mendelian Inheritance in Man (OMIM)<br/><a href='/database/62'>More</a>MIMi201450 phenotype 607008 gene

Purity/Purification    > 90% by SDS - PAGE

Form/Format    Liquid. 20mM Tris-HCl buffer (pH8.0) containing 20% glycerol 0.1M NaCl

Concentration    0.5 mg/ml (determined by Bradford assay) (lot specific)

Antigen Species    Human

Tag    His-tag

Expression System    E Coli

Preparation and Storage    Can be stored at 4 degree C short term (1-2 weeks). For long term storage, aliquot and store at -20 degree C or -70 degree C. Avoid repeated freezing and thawing cycles.

Other Notes    Small volumes of ACADM recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Product Description specifically for ACADM recombinant protein
   ACADM, also known as acyl-Coenzyme A dehydrogenase is an enzyme that is important for breaking down (degrading) a certain group of fats called medium-chain fatty acids. It is essential for converting these particular fatty acids to energy, especially during periods without food (fasting). This protein functions in mitochondria, the energy-producing centers within cells. It is found in the mitochondria of several types of tissues, particularly the liver. Recombinant human ACADM protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques

Product Categories/Family forACADM recombinant protein    Enzymes & Proteases

Applications Tested/Suitable for ACADM recombinant protein
SDS-PAGE

SDS-PAGE of ACADM recombinant protein

Sequence		MGSSHHHHHH SSGLVPRGSH MKANRQREPG LGFSFEFTEQ QKEFQATARK FAREEIIPVA AEYDKTGEYP VPLIRRAWEL GLMNTHIPEN CGGLGLGTFD ACLISEELAY GCTGVQTAIE GNSLGQMPII IAGNDQQKKK YLGRMTEEPL MCAYCVTEPG AGSDVAGIKT KAEKKGDEYI INGQKMWITN GGKANWYFLL ARSDPDPKAP ANKAFTGFIV EADTPGIQIG RKELNMGQRC SDTRGIVFED VKVPKENVLI GDGAGFKVAM GAFDKTRPVV AAGAVGLAQR ALDEATKYAL ERKTFGKLLV EHQAISFMLA EMAMKVELAR MSYQRAAWEV DSGRRNTYYA SIAKAFAGDI ANQLATDAVQ ILGGNGFNTE YPVEKLMRDA KIYQIYEGTS QIQRLIVARE HIDKYKN
OMIM		Online Mendelian Inheritance in Man (OMIM)<br/><a href='/database/62'>More</a>MIMi201450 phenotype 607008 gene


Purity/Purification		> 90% by SDS - PAGE
Form/Format		Liquid. 20mM Tris-HCl buffer (pH8.0) containing 20% glycerol 0.1M NaCl
Concentration		0.5 mg/ml (determined by Bradford assay) (lot specific)

Antigen Species		Human
Tag		His-tag
Expression System		E Coli

Preparation and Storage		Can be stored at 4 degree C short term (1-2 weeks). For long term storage, aliquot and store at -20 degree C or -70 degree C. Avoid repeated freezing and thawing cycles.
Other Notes		Small volumes of ACADM recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Product Description specifically for ACADM recombinant protein		ACADM, also known as acyl-Coenzyme A dehydrogenase is an enzyme that is important for breaking down (degrading) a certain group of fats called medium-chain fatty acids. It is essential for converting these particular fatty acids to energy, especially during periods without food (fasting). This protein functions in mitochondria, the energy-producing centers within cells. It is found in the mitochondria of several types of tissues, particularly the liver. Recombinant human ACADM protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques
Product Categories/Family forACADM recombinant protein		Enzymes & Proteases

Applications Tested/Suitable for ACADM recombinant protein		SDS-PAGE

SDS-PAGE of ACADM recombinant protein