ACAT2, 1-397aa, Human, His tag, E Coli

Instruction Manual!

Product name:	ACAT2, 1-397aa, Human, His tag, E Coli
Source:	E Coli
Purity:	>95%
Buffer Formulation:	Liquid. In 20 mM Tris-HCl buffer (pH8.0) containing 0.1M NaCl, 1mM DTT, 10% glycerol
Applications:	SDS-PAGE
Storage:	Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.
UOM:	10ug/50ug/100ug/200ug/1mg

Product Name ACAT2, Recombinant Protein
Also Known As ACAT2, 1-397aa, Human, His tag, E Coli
Product Synonym Names ACTL; Acetyl-CoA acetyltransferase; cytosolic
Product Gene Name ACAT2 recombinant protein [Similar Products]
Research Use Only For Research Use Only. Not for use in diagnostic procedures.

Sequence    MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSMNAG SDPVVIVSAA RTIIGSFNGA LAAVPVQDLG STVIKEVLKR ATVAPEDVSE VIFGHVLAAG CGQNPVRQAS VGAGIPYSVP AWSCQMICGS GLKAVCLAVQ SIGIGDSSIV VAGGMENMSK APHLAYLRTG VKIGEMPLTD SILCDGLTDA FHNCHMGITA ENVAKKWQVS REDQDKVAVL SQNRTENAQK AGHFDKEIVP VLVSTRKGLI EVKTDEFPRH GSNIEAMSKL KPYFLTDGTG TVTPANASGI NDGAAAVVLM KKSEADKRGL TPLARIVSWS QVGVEPSIMG IGPIPAIKQA VTKAGWSLED VDIFEINEAF AAVSAAIVKE LGLNPEKVNI EGGAIALGHP LGASGCRILV TLLHTLERMG RSRGVAALCI GGGMGIAMCV QRE

OMIM    Online Mendelian Inheritance in Man (OMIM)<br/><a href='/database/62'>More</a>MIMi100678 gene+phenotype

Purity/Purification    > 95% by SDS - PAGE

Form/Format    Liquid. In 20 mM Tris-HCl buffer (pH8.0) containing 0.1M NaCl, 1mM DTT, 10% glycerol

Concentration    1 mg/ml (determined by Bradford assay) (lot specific)

Antigen Species    Human

Tag    His-tag

Expression System    E Coli

Preparation and Storage    Can be stored at 4 degree C short term (1-2 weeks). For long term storage, aliquot and store at -20 degree C or -70 degree C. Avoid repeated freezing and thawing cycles.

Other Notes    Small volumes of ACAT2 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Product Description specifically for ACAT2 recombinant protein
   ACAT2 is an enzyme involved in lipid metabolism. It participates in lipoprotein assembly, catalyzing cholesterol esterification in mammalian cells. ACAT2 is an integral membrane protein that localizes to the endoplasmic reticulum of human intestinal cells. ACAT2 deficiency contributes to severe mental retardation and hypotonus. Recombinant human ACAT2 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Product Categories/Family forACAT2 recombinant protein    Enzymes & Proteases

Applications Tested/Suitable for ACAT2 recombinant protein
SDS-PAGE

SDS-PAGE of ACAT2 recombinant protein

Sequence		MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSMNAG SDPVVIVSAA RTIIGSFNGA LAAVPVQDLG STVIKEVLKR ATVAPEDVSE VIFGHVLAAG CGQNPVRQAS VGAGIPYSVP AWSCQMICGS GLKAVCLAVQ SIGIGDSSIV VAGGMENMSK APHLAYLRTG VKIGEMPLTD SILCDGLTDA FHNCHMGITA ENVAKKWQVS REDQDKVAVL SQNRTENAQK AGHFDKEIVP VLVSTRKGLI EVKTDEFPRH GSNIEAMSKL KPYFLTDGTG TVTPANASGI NDGAAAVVLM KKSEADKRGL TPLARIVSWS QVGVEPSIMG IGPIPAIKQA VTKAGWSLED VDIFEINEAF AAVSAAIVKE LGLNPEKVNI EGGAIALGHP LGASGCRILV TLLHTLERMG RSRGVAALCI GGGMGIAMCV QRE
OMIM		Online Mendelian Inheritance in Man (OMIM)<br/><a href='/database/62'>More</a>MIMi100678 gene+phenotype


Purity/Purification		> 95% by SDS - PAGE
Form/Format		Liquid. In 20 mM Tris-HCl buffer (pH8.0) containing 0.1M NaCl, 1mM DTT, 10% glycerol
Concentration		1 mg/ml (determined by Bradford assay) (lot specific)

Antigen Species		Human
Tag		His-tag
Expression System		E Coli

Preparation and Storage		Can be stored at 4 degree C short term (1-2 weeks). For long term storage, aliquot and store at -20 degree C or -70 degree C. Avoid repeated freezing and thawing cycles.
Other Notes		Small volumes of ACAT2 recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Product Description specifically for ACAT2 recombinant protein		ACAT2 is an enzyme involved in lipid metabolism. It participates in lipoprotein assembly, catalyzing cholesterol esterification in mammalian cells. ACAT2 is an integral membrane protein that localizes to the endoplasmic reticulum of human intestinal cells. ACAT2 deficiency contributes to severe mental retardation and hypotonus. Recombinant human ACAT2 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
Product Categories/Family forACAT2 recombinant protein		Enzymes & Proteases

Applications Tested/Suitable for ACAT2 recombinant protein		SDS-PAGE

SDS-PAGE of ACAT2 recombinant protein