ABO, 54-354aa, Human, His tag, E Coli

Instruction Manual!

Product name:	ABO, 54-354aa, Human, His tag, E Coli
Source:	E Coli
Purity:	>85%
Buffer Formulation:	Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 2mM DTT, 20% glycerol, 200mM NaCl
Applications:	SDS-PAGE
Storage:	Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.
UOM:	10ug/50ug/100ug/200ug/1mg

Product Name ABO, Recombinant Protein
Also Known As ABO, 54-354aa, Human, His tag, E Coli
Product Synonym Names Histo-blood group ABO system transferase; A3GALNT; A3GALT1; GTB; NAGAT; A transferase; B transferase; Fucosylglycoprotein 3-alpha-galactosyltransferase; alpha 1-3-N-acetylgalactosaminyltransferase; alpha 1-3-galactosyltransferase
Product Gene Name ABO recombinant protein [Similar Products]
Research Use Only For Research Use Only. Not for use in diagnostic procedures.
Table BarTOPTable Bar

Sequence    MGSSHHHHHH SSGLVPRGSH MAVREPDHLQ RVSLPRMVYP QPKVLTPCRK DVLVVTPWLA PIVWEGTFNI DILNEQFRLQ NTTIGLTVFA IKKYVAFLKL FLETAEKHFM VGHRVHYYVF TDQPAAVPRV TLGTGRQLSV LEVRAYKRWQ DVSMRRMEMI SDFCERRFLS EVDYLVCVDV DMEFRDHVGV EILTPLFGTL HPGFYGSSRE AFTYERRPQS QAYIPKDEGD FYYLGGFFGG SVQEVQRLTR ACHQAMMVDQ ANGIEAVWHD ESHLNKYLLR HKPTKVLSPE YLWDQQLLGW PAVLRKLRFT AVPKNHQAVR NP

OMIM    Online Mendelian Inheritance in Man (OMIM)<br/><a href='/database/62'>More</a>MIMi110300 gene

Purity/Purification    > 85% by SDS - PAGE

Form/Format    Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 2mM DTT, 20% glycerol, 200mM NaCl

Concentration    0.5mg/ml (determined by Bradford assay) (lot specific)

Antigen Species    Human

Tag    His-tag

Expression System    E Coli

Preparation and Storage    Can be stored at 4 degree C short term (1-2 weeks). For long term storage, aliquot and store at -20 degree C or -70 degree C. Avoid repeated freezing and thawing cycles.

Other Notes    Small volumes of ABO recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Product Description specifically for ABO recombinant protein
   ABO, also known as NAGAT, belongs to the glycosyltransferase 6 family. This protein is the basis of the ABO blood group system and related to the first discovered blood group system, ABO. Which allele is present in an individual determines the blood group. The histo-blood group ABO involves three carbohydrate antigens: A, B, and H. A, B, and AB individuals express a glycosyltransferase activity that converts the H antigen to the A antigen (by addition of uDP-GalNAc) or to the B antigen (by addition of uDP-Gal), whereas O individuals lack such activity. Recombinant human ABO protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Product Categories/Family forABO recombinant protein    Enzymes & Proteases

Applications Tested/Suitable for ABO recombinant protein
SDS-PAGE

SDS-PAGE of ABO recombinant protein

Sequence		MGSSHHHHHH SSGLVPRGSH MAVREPDHLQ RVSLPRMVYP QPKVLTPCRK DVLVVTPWLA PIVWEGTFNI DILNEQFRLQ NTTIGLTVFA IKKYVAFLKL FLETAEKHFM VGHRVHYYVF TDQPAAVPRV TLGTGRQLSV LEVRAYKRWQ DVSMRRMEMI SDFCERRFLS EVDYLVCVDV DMEFRDHVGV EILTPLFGTL HPGFYGSSRE AFTYERRPQS QAYIPKDEGD FYYLGGFFGG SVQEVQRLTR ACHQAMMVDQ ANGIEAVWHD ESHLNKYLLR HKPTKVLSPE YLWDQQLLGW PAVLRKLRFT AVPKNHQAVR NP
OMIM		Online Mendelian Inheritance in Man (OMIM)<br/><a href='/database/62'>More</a>MIMi110300 gene


Purity/Purification		> 85% by SDS - PAGE
Form/Format		Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 2mM DTT, 20% glycerol, 200mM NaCl
Concentration		0.5mg/ml (determined by Bradford assay) (lot specific)

Antigen Species		Human
Tag		His-tag
Expression System		E Coli

Preparation and Storage		Can be stored at 4 degree C short term (1-2 weeks). For long term storage, aliquot and store at -20 degree C or -70 degree C. Avoid repeated freezing and thawing cycles.
Other Notes		Small volumes of ABO recombinant protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Product Description specifically for ABO recombinant protein		ABO, also known as NAGAT, belongs to the glycosyltransferase 6 family. This protein is the basis of the ABO blood group system and related to the first discovered blood group system, ABO. Which allele is present in an individual determines the blood group. The histo-blood group ABO involves three carbohydrate antigens: A, B, and H. A, B, and AB individuals express a glycosyltransferase activity that converts the H antigen to the A antigen (by addition of uDP-GalNAc) or to the B antigen (by addition of uDP-Gal), whereas O individuals lack such activity. Recombinant human ABO protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
Product Categories/Family forABO recombinant protein		Enzymes & Proteases

Applications Tested/Suitable for ABO recombinant protein		SDS-PAGE

SDS-PAGE of ABO recombinant protein