Eukaryotic Green fluorescent protein (GFP)

[ PROPERTIES ]

Source: Eukaryotic expression.

Host: Yeast

Residues: Met1~Lys238

Tags: N-terminal His Tag

Homology: Mouse 100%, rat 100%

Subcellular Location: Secreted.

Purity: >95%

Endotoxin Level: <1.0EU per 1μg (determined by the LAL method).

Traits: Freeze-dried powder

Buffer Formulation: 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 5%Trehalose and Proclin300.

Original Concentration: 200ug/mL

Predicted isoelectric point: 5.7

Predicted Molecular Mass: 27.1kDa

Accurate Molecular Mass: 28kDa as determined by SDS-PAGE reducing conditions.

Applications: SDS-PAGE; WB; ELISA; IP; CoIP; EMSA; Purification; Amine Reactive Labeling.

(May be suitable for use in other assays to be determined by the end user.)

[ USAGE ]

Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex

[ STORAGE AND STABILITY ]

Storage: Avoid repeated freeze/thaw cycles. Store at 2-8oC for one month.

Aliquot and store at -80^oC for 12 months.

Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, and no obvious degradation and precipitation were observed.The loss rate is less than 5% within the expiration date under appropriate storage condition.

[ SEQUENCE ]

[ IDENTIFICATION ]